TRPP3 / RacK1

Interaction: TRPP3Detail / RacK1Detail
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Screening Toggle
  Experimental screening Non-experimental screening Reference
TRP channel construct Interactor source
TRP channel Interactor Method Species Region Species Organ/tissue Sample type
TRPP3 RACK1 Yeast two-hybrid Human 1-291 Human Kidney cDNA library 22174419
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Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Validation: In vitro validation Toggle
  Assay with recombinant proteins Reference
TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Expression system Species Region Expression system
TRPP3 RACK1 Fusion protein-pull down assay Human 1-291 HEK293 lysates Human Full-length E. coli 22174419
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Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Validation: In vivo validation Toggle
  Assay with endogenous proteins Assay with overexpressed proteins Reference
Cell or tissue Cell or tissue TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Species Region
TRPP3 RACK1 Co-immunoprecipitation Xenopus oocyte Human Full-length Human Full-length 22174419
TRPP3 RACK1 Co-immunoprecipitation HEK293 Human Full-length Human Full-length 22174419
TRPP3 RACK1 Co-immunoprecipitation IMCD3 Human Full-length Human Full-length 22174419
TRPP3 RACK1 Co-immunoprecipitation Mouse kidney lysates 22174419
TRPP3 RACK1 Co-immunoprecipitation Mouse heart tissue lysates 22174419
TRPP3 RACK1 Co-immunoprecipitation Mouse brain tissue lysates 22174419
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Characterization Toggle
  Binding region mapping Stoichiometry Affinity (Kd) Reference
TRP channel Interactor
TRP channel Interactor Method Species Region Species Region
TRPP3 RACK1 Yeast two-hybrid Human 1-291 Human 415-669 22174419
TRPP3 RACK1 Yeast two-hybrid Human 1-291 Human 649-951 22174419
TRPP3 RACK1 Yeast two-hybrid Human 55-291 Human 415-951 22174419
TRPP3 RACK1 Yeast two-hybrid Human 1696-2415 Human Not specified 22174419
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Functional consequence Toggle
TRP channel Interactor Method Post-translational modification Subcellular trafficking Activity Reference
TRPP3 RACK1 Calcium measurement Inhibition 22174419
TRPP3 RACK1 Cell surface biotinylation Decrease in plasma membrane level 22174419
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
TRP / Interactor

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