TRPV1 / PRKD1/PKCμ
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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Screening
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| Experimental screening | Non-experimental screening | Reference | |||||||
| TRP channel construct | Interactor source | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | ||
| TRPV1 | PRKD1/PKCμ | Inference | Prediction | 15471852 | |||||
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Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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Validation: In vitro validation
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| Assay with recombinant proteins | Reference | ||||||||
| TRP channel construct | Interactor construct | ||||||||
| TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | |
| TRPV1 | PRKD1/PKCμ | Fusion protein-pull down assay | Rat | Full-length | E. coli | Not specified | Full-length | Not specified | 15471852 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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|
Validation: In vivo validation
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| Assay with endogenous proteins | Assay with overexpressed proteins | Reference | |||||||
| Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | ||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPV1 | PRKD1/PKCμ | Co-immunofluorescence staining | Rat dorsal root ganglion section | 18063480 | |||||
| TRPV1 | PRKD1/PKCμ | Co-immunoprecipitation | Rat dorsal root ganglion lysates | 18063480 | |||||
| TRPV1 | PRKD1/PKCμ | Co-immunoprecipitation | CHO | Rat | Full-length | Human | Full-length | 15471852 | |
| TRPV1 | PRKD1/PKCμ | Co-immunoprecipitation | Rat dorsal root ganglion neuron | 15471852 | |||||
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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Characterization
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| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | ||||||
| TRP channel | Interactor | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPV1 | PRKD1/PKCμ | Fusion protein-pull down assay | Rat | 99-118 | Human | Not determined | 15471852 | ||
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
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Functional consequence
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| TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | |||||
| TRPV1 | PRKD1/PKCμ | Calcium measurement | Activation | 15471852 | |||||||
| TRPV1 | PRKD1/PKCμ | In vitro PTM assay | Phosphorylation (Ser-116) | 15471852 | |||||||
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)




Screening