ơ-actinin-1 / TRP channels
Name:
ơ-actinin-1
*
UniProtKB IDs are not shown, 1) if protein (or gene) isotypes are not specified in the referred articles, 2) if their IDs are not found in the UniprotKB database or by us
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External DB
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| Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM | |
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| Mouse | ||||||||||
| Rat | ||||||||||
Biological Process:
No information in Gene Ontology
Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
World Wide Web URL: http://www.geneontology.org/
Disease:
No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine,
Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/
World Wide Web URL: http://omim.org/
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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Screening
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| Experimental screening | Non-experimental screening | Reference | ||||||||
| TRP channel construct | Interactor source | |||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | |||
| TRPC1 |
|
ơ-actinin-1 | Inference | Prediction | 17892531 | |||||
| TRPC5 |
|
ơ-actinin-1 | Affinity purification-mass spectrometry | Endogenous protein | Rat | Brain | Cerebral cortex lysates | 16025302 | ||
| TRPP1 |
|
ơ-actinin-1 | Inference | Prediction | 15843396 | |||||
| TRPP2 |
|
ơ-actinin-1 | Inference | Prediction | 17944866 | |||||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Validation: In vitro validation
|
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|---|---|---|---|---|---|---|---|---|---|---|
| Assay with recombinant proteins | Reference | |||||||||
| TRP channel construct | Interactor construct | |||||||||
| TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | ||
| TRPP1 |
|
ơ-actinin-1 | Fusion protein-pull down assay | Human | 1-215 | E. coli | Chicken | Not specified | Not specified | 15843396 |
| TRPP1 |
|
ơ-actinin-1 | Fusion protein-pull down assay | Human | 821-968 | E. coli | Chicken | Not specified | Not specified | 15843396 |
| TRPP2 |
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ơ-actinin-1 | Yeast two-hybrid | Human | 1-97 | Yeast | Human | 334-892 | Yeast | 17944866 |
| TRPP2 |
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ơ-actinin-1 | Yeast two-hybrid | Human | 562-805 | Yeast | Human | 334-892 | Yeast | 17944866 |
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Validation: In vivo validation
|
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|---|---|---|---|---|---|---|---|---|---|---|
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference | ||||||||
| Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | |||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
| TRPC1 |
|
ơ-actinin-1 | Co-immunoprecipitation | Human platelets | 17892531 | |||||
| TRPP1 |
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ơ-actinin-1 | Co-immunofluorescence staining | NIH-3T3 | 15843396 | |||||
| TRPP1 |
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ơ-actinin-1 | Co-immunofluorescence staining | MDCK | 15843396 | |||||
| TRPP1 |
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ơ-actinin-1 | Co-immunofluorescence staining | Mouse inner medullary collecting duct cell | 15843396 | |||||
| TRPP1 |
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ơ-actinin-1 | Co-immunoprecipitation | HEK293 | 15843396 | |||||
| TRPP1 |
|
ơ-actinin-1 | Co-immunoprecipitation | MDCK | 15843396 | |||||
| TRPP1 |
|
ơ-actinin-1 | Co-immunoprecipitation | Rat kidney tissue | 15843396 | |||||
| TRPP2 |
|
ơ-actinin-1 | Co-immunoprecipitation | HEK293 | 17944866 | |||||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Characterization
|
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|---|---|---|---|---|---|---|---|---|---|---|
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | |||||||
| TRP channel | Interactor | |||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
| TRPP1 |
|
ơ-actinin-1 | Fusion protein-pull down assay | Human | 821-878 | Chicken | Not determined | 15843396 | ||
| TRPP1 |
|
ơ-actinin-1 | Yeast two-hybrid | Human | 821-878 | Human | 334-892 | 15843396 | ||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)