β-tubulin-5 / TRP channels

Name: Tubulin beta chain (β-tubulin-5)
Official Symbol: TUBB provided by HGNC
Function:
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).
Species External DB Questionmark
Entrez Gene UniprotKB DIP IntAct MINT BioGRID STRING IUPHAR-DB KEGG OMIM
Human
Seq		 203068External P07437External DIP-32772NExternal P07437External MINT-1146393External 128444External P07437External   hsa:203068External 191130External
Mouse
Seq		 22154External P99024External DIP-33914NExternal P99024External MINT-1869561External 204381External P99024External   mmu:22154External  
Rat
Seq		 29214External P69897External DIP-37355NExternal P69897External MINT-2513334External 247892External P69897External   rno:29214External  
PPI pairs:
Biological Process:
Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Screening Toggle
  Experimental screening Non-experimental screening Reference
TRP channel construct Interactor source
TRP channel Interactor Method Species Region Species Organ/tissue Sample type
TRPC1 Link β-tubulin-5 Affinity purification-mass spectrometry Human Full-length Bovine Retinal Tissue lysates 15935109
TRPC1 Link β-tubulin-5 Yeast two-hybrid Human 1-139 Rat Brain cDNA library 15935109
TRPV1 Link β-tubulin-5 Affinity purification-mass spectrometry Rat 681-838 Rat Spinal cord Extract 15569253
TRPV4 Link β-tubulin-5 Inference Prediction 22309793
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Validation: In vitro validation Toggle
  Assay with recombinant proteins Reference
TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Expression system Species Region Expression system
TRPV1 Link β-tubulin-5 Fusion protein-pull down assay Rat 681-838 E. coli Rat Full-length Rat spinal cord extract 15569253
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Validation: In vivo validation Toggle
  Assay with endogenous proteins Assay with overexpressed proteins Reference
Cell or tissue Cell or tissue TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Species Region
TRPC1 Link β-tubulin-5 Co-immunofluorescence staining ARPE-19 Human Full-length Not used 15935109
TRPC1 Link β-tubulin-5 Co-immunoprecipitation ARPE-19 Human Full-length Not used 15935109
TRPV1 Link β-tubulin-5 Co-immunoprecipitation Rat dorsal root ganglion derived cell line F11 Rat Full-length Not used 15569253
TRPV4 Link β-tubulin-5 Co-immunofluorescence staining HEK293 Rat Full-length Rat Full-length 22309793
TRPV4 Link β-tubulin-5 Co-immunoprecipitation HEK293 Rat Full-length Rat Full-length 22309793
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Functional consequence Toggle
TRP channel Interactor Method Post-translational modification Subcellular trafficking Activity Reference
TRPC1 Link β-tubulin-5 Calcium measurement Inhibition 15935109
TRPC1 Link β-tubulin-5 Co-immunofluorescence staining Increase in plasma membrane level 15935109
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
TRP / Interactor

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