AKAP5/AKAP150 / TRP channels
Name: A-kinase anchor protein 5
(AKAP5/AKAP150)
Official Symbol: AKAP5
provided by HGNC
Function:
May anchor the PKA protein to cytoskeletal and/or organelle-associated proteins, targeting the signal carried by cAMP to specific intracellular effectors. Association with to the beta2-adrenergic receptor (beta2-AR) not only regulates beta2-AR signaling pathway, but also the activation by PKA by switching off the beta2-AR signaling cascade.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt)
Nucleic Acids Res. 40: D71-D75 (2012).
Species |
External DB
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Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM | |
Human
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9495![]() |
P24588![]() |
DIP-186N![]() |
P24588![]() |
MINT-1733931![]() |
114874![]() |
P24588![]() |
hsa:9495![]() |
604688![]() |
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Mouse
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238276![]() |
D3YVF0![]() |
231964![]() |
mmu:238276![]() |
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Rat
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171026![]() |
P24587![]() |
UNK-5374438![]() |
251053![]() |
P24587![]() |
rno:171026![]() |
Biological Process:
- GO:0043624
: cellular protein complex disassembly
- GO:0070887
: cellular response to chemical stimulus
- GO:0006112
: energy reserve metabolic process
- GO:0021766
: hippocampus development
- GO:0060135
: maternal process involved in female pregnancy
- GO:0043267
: negative regulation of potassium ion transport
- GO:0010524
: positive regulation of calcium ion transport into cytosol
- GO:0030819
: positive regulation of cAMP biosynthetic process
- GO:0050775
: positive regulation of dendrite morphogenesis
- GO:0051533
: positive regulation of NFAT protein import into nucleus
- GO:0010739
: positive regulation of protein kinase A signaling cascade
- GO:0001934
: positive regulation of protein phosphorylation
- GO:0006605
: protein targeting
- GO:0050796
: regulation of insulin secretion
- GO:0051602
: response to electrical stimulus
- GO:0014850
: response to muscle activity
- GO:0007165
: signal transduction
- GO:0044281
: small molecule metabolic process
- GO:0021510
: spinal cord development
- GO:0007268
: synaptic transmission
Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
World Wide Web URL: http://www.geneontology.org/
Disease:
No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine,
Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/
World Wide Web URL: http://omim.org/
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Screening
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Experimental screening | Non-experimental screening | Reference | ||||||||
TRP channel construct | Interactor source | |||||||||
TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | |||
TRPA1 |
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AKAP5/AKAP150 | Speculation | Prediction | 18701070 | |||||
TRPM8 |
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AKAP5/AKAP150 | Speculation | Prediction | 18701070 | |||||
TRPV1 |
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AKAP5/AKAP150 | Inference | Prediction | 18381233 | |||||
TRPV2 |
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AKAP5/AKAP150 | Speculation | Prediction | 18701070 | |||||
TRPV3 |
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AKAP5/AKAP150 | Speculation | Prediction | 18701070 | |||||
TRPV4 |
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AKAP5/AKAP150 | Speculation | Prediction | 18701070 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Validation: In vitro validation
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Assay with recombinant proteins | Reference | |||||||||
TRP channel construct | Interactor construct | |||||||||
TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | ||
TRPV1 |
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AKAP5/AKAP150 | Fusion protein-pull down assay | Human | 681-839 | E. coli | Not used | HEK293 lysates | 18701070 |
(
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Validation: In vivo validation
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Assay with endogenous proteins | Assay with overexpressed proteins | Reference | ||||||||
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | |||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
TRPA1 |
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AKAP5/AKAP150 | Co-immunoprecipitation | HEK293 | Mouse | Full-length | Not used | 18701070 | ||
TRPM8 |
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AKAP5/AKAP150 | Co-immunoprecipitation | HEK293 | Rat | Full-length | Not used | 18701070 | ||
TRPV1 |
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AKAP5/AKAP150 | Co-immunofluorescence staining | Rat trigeminal ganglia neuron | 18381233 | |||||
TRPV1 |
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AKAP5/AKAP150 | Co-immunofluorescence staining | Mouse dorsal root ganglion tissue | 18463244 | |||||
TRPV1 |
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AKAP5/AKAP150 | Co-immunofluorescence staining | Rat dorsal root ganglion neuron | 18701070 | |||||
TRPV1 |
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AKAP5/AKAP150 | Co-immunoprecipitation | Rat trigeminal ganglia neuron | 22789851 | |||||
TRPV1 |
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AKAP5/AKAP150 | Co-immunoprecipitation | Rat trigeminal ganglia neuron | 18381233 | |||||
TRPV1 |
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AKAP5/AKAP150 | Co-immunoprecipitation | CHO | Rat | Full-length | Rat | Full-length | 18381233 | |
TRPV1 |
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AKAP5/AKAP150 | Co-immunoprecipitation | Mouse spinalcord tissue | 18463244 | |||||
TRPV1 |
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AKAP5/AKAP150 | Co-immunoprecipitation | Mouse dorsal root ganglion tissue | 18463244 | |||||
TRPV1 |
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AKAP5/AKAP150 | Co-immunoprecipitation | HEK293 | Human | Full-length | Human | Full-length | 23264624 | |
TRPV1 |
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AKAP5/AKAP150 | Co-immunoprecipitation | HEK293 | Human | Full-length | Mouse | Full-length | 23264624 | |
TRPV1 |
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AKAP5/AKAP150 | Co-immunoprecipitation | Rat dorsal root ganglion neuron | 18701070 | |||||
TRPV1 |
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AKAP5/AKAP150 | Co-immunoprecipitation | HEK293 | Human | Full-length | Not used | 18701070 | ||
TRPV2 |
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AKAP5/AKAP150 | Co-immunoprecipitation | HEK293 | Human | Full-length | Not used | 18701070 | ||
TRPV3 |
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AKAP5/AKAP150 | Co-immunoprecipitation | HEK293 | Mouse | Full-length | Not used | 18701070 | ||
TRPV4 |
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AKAP5/AKAP150 | Co-immunofluorescence staining | Rat cerebral artery | 24778429 | |||||
TRPV4 |
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AKAP5/AKAP150 | Co-immunoprecipitation | HEK293 | Human | Full-length | Not used | 18701070 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Characterization
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Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | |||||||
TRP channel | Interactor | |||||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
TRPV1 |
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AKAP5/AKAP150 | Co-immunoprecipitation | Human | Not determined | Human | 326-336 | 23699529 | ||
TRPV1 |
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AKAP5/AKAP150 | Co-immunoprecipitation | Human | 736-749 | Human | Not determined | 18701070 | ||
TRPV1 |
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AKAP5/AKAP150 | Fluorescence resonance energy transfer | Human | Not determined | Human | 326-336 | 23699529 | ||
TRPV1 |
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AKAP5/AKAP150 | Fusion protein-pull down assay | Human | 736-749 | Human | Not determined | 18701070 | ||
TRPV1 |
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AKAP5/AKAP150 | Patch clamp | Human | 738 ?745 | Human | Not determined | 23616546 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Functional consequence
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TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | ||||||
TRPV1 |
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AKAP5/AKAP150 | Cell surface biotinylation | Increase in plasma membrane level | 18701070 | |||||||
TRPV1 |
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AKAP5/AKAP150 | Co-immunofluorescence staining | Increase in plasma membrane level | 23699529 | |||||||
TRPV1 |
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AKAP5/AKAP150 | Co-immunoprecipitation | Increase in plasma membrane level | 23616546 | |||||||
TRPV1 |
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AKAP5/AKAP150 | In vivo PTM assay | Phosphorylation | 18381233 | |||||||
TRPV1 |
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AKAP5/AKAP150 | Patch clamp | Activation | 18701070 | |||||||
TRPV4 |
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AKAP5/AKAP150 | Patch clamp | Activation | 25005230 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
