AKAP5/AKAP150 / TRP channels
Name: A-kinase anchor protein 5
(AKAP5/AKAP150)
Official Symbol: AKAP5
provided by HGNC
Function:
May anchor the PKA protein to cytoskeletal and/or organelle-associated proteins, targeting the signal carried by cAMP to specific intracellular effectors. Association with to the beta2-adrenergic receptor (beta2-AR) not only regulates beta2-AR signaling pathway, but also the activation by PKA by switching off the beta2-AR signaling cascade.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt)
Nucleic Acids Res. 40: D71-D75 (2012).
| Species |
External DB
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM | |
Human
|
9495 |
P24588 |
DIP-186N |
P24588 |
MINT-1733931 |
114874 |
P24588 |
hsa:9495 |
604688 |
|
|
Mouse
|
238276 |
D3YVF0 |
231964 |
mmu:238276 |
||||||
|
Rat
|
171026 |
P24587 |
UNK-5374438 |
251053 |
P24587 |
rno:171026 |
||||
Biological Process:
- GO:0043624
: cellular protein complex disassembly - GO:0070887 : cellular response to chemical stimulus
- GO:0006112 : energy reserve metabolic process
- GO:0021766 : hippocampus development
- GO:0060135 : maternal process involved in female pregnancy
- GO:0043267 : negative regulation of potassium ion transport
- GO:0010524 : positive regulation of calcium ion transport into cytosol
- GO:0030819 : positive regulation of cAMP biosynthetic process
- GO:0050775 : positive regulation of dendrite morphogenesis
- GO:0051533 : positive regulation of NFAT protein import into nucleus
- GO:0010739 : positive regulation of protein kinase A signaling cascade
- GO:0001934 : positive regulation of protein phosphorylation
- GO:0006605 : protein targeting
- GO:0050796 : regulation of insulin secretion
- GO:0051602 : response to electrical stimulus
- GO:0014850 : response to muscle activity
- GO:0007165 : signal transduction
- GO:0044281 : small molecule metabolic process
- GO:0021510 : spinal cord development
- GO:0007268 : synaptic transmission
Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
World Wide Web URL: http://www.geneontology.org/
Disease:
No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine,
Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/
World Wide Web URL: http://omim.org/
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
Screening
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Experimental screening | Non-experimental screening | Reference | ||||||||
| TRP channel construct | Interactor source | |||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | |||
| TRPA1 |
|
AKAP5/AKAP150 | Speculation | Prediction | 18701070 | |||||
| TRPM8 |
|
AKAP5/AKAP150 | Speculation | Prediction | 18701070 | |||||
| TRPV1 |
|
AKAP5/AKAP150 | Inference | Prediction | 18381233 | |||||
| TRPV2 |
|
AKAP5/AKAP150 | Speculation | Prediction | 18701070 | |||||
| TRPV3 |
|
AKAP5/AKAP150 | Speculation | Prediction | 18701070 | |||||
| TRPV4 |
|
AKAP5/AKAP150 | Speculation | Prediction | 18701070 | |||||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Validation: In vitro validation
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Assay with recombinant proteins | Reference | |||||||||
| TRP channel construct | Interactor construct | |||||||||
| TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | ||
| TRPV1 |
|
AKAP5/AKAP150 | Fusion protein-pull down assay | Human | 681-839 | E. coli | Not used | HEK293 lysates | 18701070 | |
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Validation: In vivo validation
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference | ||||||||
| Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | |||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
| TRPA1 |
|
AKAP5/AKAP150 | Co-immunoprecipitation | HEK293 | Mouse | Full-length | Not used | 18701070 | ||
| TRPM8 |
|
AKAP5/AKAP150 | Co-immunoprecipitation | HEK293 | Rat | Full-length | Not used | 18701070 | ||
| TRPV1 |
|
AKAP5/AKAP150 | Co-immunofluorescence staining | Rat trigeminal ganglia neuron | 18381233 | |||||
| TRPV1 |
|
AKAP5/AKAP150 | Co-immunofluorescence staining | Mouse dorsal root ganglion tissue | 18463244 | |||||
| TRPV1 |
|
AKAP5/AKAP150 | Co-immunofluorescence staining | Rat dorsal root ganglion neuron | 18701070 | |||||
| TRPV1 |
|
AKAP5/AKAP150 | Co-immunoprecipitation | Rat trigeminal ganglia neuron | 22789851 | |||||
| TRPV1 |
|
AKAP5/AKAP150 | Co-immunoprecipitation | Rat trigeminal ganglia neuron | 18381233 | |||||
| TRPV1 |
|
AKAP5/AKAP150 | Co-immunoprecipitation | CHO | Rat | Full-length | Rat | Full-length | 18381233 | |
| TRPV1 |
|
AKAP5/AKAP150 | Co-immunoprecipitation | Mouse spinalcord tissue | 18463244 | |||||
| TRPV1 |
|
AKAP5/AKAP150 | Co-immunoprecipitation | Mouse dorsal root ganglion tissue | 18463244 | |||||
| TRPV1 |
|
AKAP5/AKAP150 | Co-immunoprecipitation | HEK293 | Human | Full-length | Human | Full-length | 23264624 | |
| TRPV1 |
|
AKAP5/AKAP150 | Co-immunoprecipitation | HEK293 | Human | Full-length | Mouse | Full-length | 23264624 | |
| TRPV1 |
|
AKAP5/AKAP150 | Co-immunoprecipitation | Rat dorsal root ganglion neuron | 18701070 | |||||
| TRPV1 |
|
AKAP5/AKAP150 | Co-immunoprecipitation | HEK293 | Human | Full-length | Not used | 18701070 | ||
| TRPV2 |
|
AKAP5/AKAP150 | Co-immunoprecipitation | HEK293 | Human | Full-length | Not used | 18701070 | ||
| TRPV3 |
|
AKAP5/AKAP150 | Co-immunoprecipitation | HEK293 | Mouse | Full-length | Not used | 18701070 | ||
| TRPV4 |
|
AKAP5/AKAP150 | Co-immunofluorescence staining | Rat cerebral artery | 24778429 | |||||
| TRPV4 |
|
AKAP5/AKAP150 | Co-immunoprecipitation | HEK293 | Human | Full-length | Not used | 18701070 | ||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Characterization
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | |||||||
| TRP channel | Interactor | |||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
| TRPV1 |
|
AKAP5/AKAP150 | Co-immunoprecipitation | Human | Not determined | Human | 326-336 | 23699529 | ||
| TRPV1 |
|
AKAP5/AKAP150 | Co-immunoprecipitation | Human | 736-749 | Human | Not determined | 18701070 | ||
| TRPV1 |
|
AKAP5/AKAP150 | Fluorescence resonance energy transfer | Human | Not determined | Human | 326-336 | 23699529 | ||
| TRPV1 |
|
AKAP5/AKAP150 | Fusion protein-pull down assay | Human | 736-749 | Human | Not determined | 18701070 | ||
| TRPV1 |
|
AKAP5/AKAP150 | Patch clamp | Human | 738 ?745 | Human | Not determined | 23616546 | ||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Functional consequence
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | ||||||
| TRPV1 |
|
AKAP5/AKAP150 | Cell surface biotinylation | Increase in plasma membrane level | 18701070 | |||||||
| TRPV1 |
|
AKAP5/AKAP150 | Co-immunofluorescence staining | Increase in plasma membrane level | 23699529 | |||||||
| TRPV1 |
|
AKAP5/AKAP150 | Co-immunoprecipitation | Increase in plasma membrane level | 23616546 | |||||||
| TRPV1 |
|
AKAP5/AKAP150 | In vivo PTM assay | Phosphorylation | 18381233 | |||||||
| TRPV1 |
|
AKAP5/AKAP150 | Patch clamp | Activation | 18701070 | |||||||
| TRPV4 |
|
AKAP5/AKAP150 | Patch clamp | Activation | 25005230 | |||||||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)