Gαq / TRP channels

Name: Guanine nucleotide-binding protein G(q) subunit alpha (Gαq)
Official Symbol: GNAQ provided by HGNC
Function:
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Regulates B-cell selection and survival and is required to prevent B-cell-dependent autoimmunity. Regulates chemotaxis of BM-derived neutrophils and dendritic cells (in vitro) (By similarity).
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).
Species External DB Questionmark
Entrez Gene UniprotKB DIP IntAct MINT BioGRID STRING IUPHAR-DB KEGG OMIM
Human
Seq		 2776External P50148External DIP-41652NExternal P50148External MINT-262439External 109038External P50148External   hsa:2776External 600998External
Mouse
Seq		 14682External P21279External DIP-606NExternal P21279External MINT-4096294External 199971External P21279External   mmu:14682External  
Rat
Seq		 81666External P82471External       249565External P82471External   rno:81666External  
PPI pairs:
Biological Process:
Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Screening Toggle
  Experimental screening Non-experimental screening Reference
TRP channel construct Interactor source
TRP channel Interactor Method Species Region Species Organ/tissue Sample type
TRPC3 Link Gαq Affinity purification-mass spectrometry Not used as a bait Rat Brain Crude membranes 18205297
TRPM8 Link Gαq Inference Prediction 22460725
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Validation: In vitro validation Toggle
  Assay with recombinant proteins Reference
TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Expression system Species Region Expression system
TRPM8 Link Gαq Fusion protein-pull down assay Human Full-length HEK293 lysates Human Full-length HEK293 lysates 22750945
TRPM8 Link Gαq Fusion protein-pull down assay Human Full-length E. coli Human Full-length HEK293 lysates 22750945
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Validation: In vivo validation Toggle
  Assay with endogenous proteins Assay with overexpressed proteins Reference
Cell or tissue Cell or tissue TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Species Region
TRPM8 Link Gαq Co-immunofluorescence staining HEK293 Not specified Full-length Not specified Full-length 22460725
TRPM8 Link Gαq Co-immunoprecipitation Mouse dorsal root ganglion cell 22750945
TRPM8 Link Gαq Fluorescence resonance energy transfer HEK293 Not specified Full-length Not specified Full-length 22460725
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Characterization Toggle
  Binding region mapping Stoichiometry Affinity (Kd) Reference
TRP channel Interactor
TRP channel Interactor Method Species Region Species Region
TRPM8 Link Gαq Fusion protein-pull down assay Human 1-691 Human Not determined 22750945
TRPM8 Link Gαq Fusion protein-pull down assay Human 980-1104 Human Not determined 22750945
TRPM8 Link Gαq Fusion protein-pull down assay Human Not determined Human 222-245 22750945
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Functional consequence Toggle
TRP channel Interactor Method Post-translational modification Subcellular trafficking Activity Reference
TRPM8 Link Gαq Patch clamp Inhibition 22750945
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
TRP / Interactor

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