Homer-1 / TRP channels

Name: Homer protein homolog 1 (Homer-1)
Official Symbol: HOMER1 provided by HGNC
Function:
Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).
Species External DB Questionmark
Entrez Gene UniprotKB DIP IntAct MINT BioGRID STRING IUPHAR-DB KEGG OMIM
Human
Seq		 9456External Q86YM7External DIP-42866NExternal Q86YM7External MINT-1481241External 114845External Q86YM7External   hsa:9456External 604798External
Mouse
Seq		 26556External Q9Z2Y3External DIP-31580NExternal Q9Z2Y3External MINT-3370083External 205014External Q9Z2Y3External   mmu:26556External  
Rat
Seq		 29546External Q9Z214External DIP-44898NExternal Q9Z214External MINT-1891037External 248180External Q9Z214External   rno:29546External  
PPI pairs:
Biological Process:
  • GO:0007206 External : activation of phospholipase C activity by G-protein coupled glutamate receptor signaling pathway
  • GO:2001256 External : regulation of store-operated calcium entry
  • GO:0051592 External : response to calcium ion
  • GO:0007268 External : synaptic transmission
Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Screening Toggle
  Experimental screening Non-experimental screening Reference
TRP channel construct Interactor source
TRP channel Interactor Method Species Region Species Organ/tissue Sample type
TRPC1 Link Homer-1 Inference Prediction 14505576
TRPC2 Link Homer-1 Inference Prediction 14505576
TRPC3 Link Homer-1 Inference Prediction 16887806
TRPC5 Link Homer-1 Inference Prediction 14505576
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Validation: In vitro validation Toggle
  Assay with recombinant proteins Reference
TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Expression system Species Region Expression system
TRPC3 Link Homer-1 Fusion protein-pull down assay Human Full-length HEK293 lysates Human Full-length E. coli 16887806
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Validation: In vivo validation Toggle
  Assay with endogenous proteins Assay with overexpressed proteins Reference
Cell or tissue Cell or tissue TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Species Region
TRPC1 Link Homer-1 Co-immunoprecipitation Human platelets 22506990
TRPC1 Link Homer-1 Co-immunoprecipitation HEK293 Human Full-length Not used 14505576
TRPC1 Link Homer-1 Co-immunoprecipitation Rat brain hippocampus 14505576
TRPC1 Link Homer-1 Co-immunoprecipitation Rat astrocyte 16905188
TRPC2 Link Homer-1 Co-immunoprecipitation HEK293 Mouse Full-length Not used 14505576
TRPC2 Link Homer-1 Co-immunoprecipitation Rat vomeronasal organ lysates 20492691
TRPC5 Link Homer-1 Co-immunoprecipitation HEK293 Mouse Full-length Not used 14505576
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Functional consequence Toggle
TRP channel Interactor Method Post-translational modification Subcellular trafficking Activity Reference
TRPC3 Link Homer-1 Cell surface biotinylation Increase in plasma membrane level 16887806
TRPC3 Link Homer-1 Patch clamp Activation 16887806
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
TRP / Interactor

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