NHERF1 / TRP channels

Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF1 (NHERF1)
Official Symbol: SLC9A3R1 provided by HGNC
Function:
Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Interacts with MCC.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).
Species External DB Questionmark
Entrez Gene UniprotKB DIP IntAct MINT BioGRID STRING IUPHAR-DB KEGG OMIM
Human
Seq		 9368External O14745External DIP-29092NExternal O14745External MINT-4998796External 114769External O14745External   hsa:9368External 604990External
Mouse
Seq		 26941External P70441External DIP-38078NExternal P70441External MINT-144615External 205077External P70441External   mmu:26941External  
Rat
Seq		 59114External Q9JJ19External DIP-35760NExternal Q9JJ19External MINT-4568572External 859506External Q9JJ19External   rno:59114External  
PPI pairs:
Biological Process:
Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease:
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Screening Toggle
  Experimental screening Non-experimental screening Reference
TRP channel construct Interactor source
TRP channel Interactor Method Species Region Species Organ/tissue Sample type
TRPC4 Link NHERF1 Inference Prediction 10980202
TRPC4 Link NHERF1 Yeast two-hybrid Human 686-977 Human Brain cDNA library 18048348
TRPC5 Link NHERF1 Inference Prediction 10980202
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Validation: In vitro validation Toggle
  Assay with recombinant proteins Reference
TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Expression system Species Region Expression system
TRPC4 Link NHERF1 Fusion protein-pull down assay Mouse Full-length In vitro translation Mouse 1-107 E. coli 10980202
TRPC4 Link NHERF1 Fusion protein-pull down assay Human Full-length HEK293 lysates Human 1-102 E. coli 12154080
TRPC4 Link NHERF1 Fusion protein-pull down assay Human Full-length HEK293 lysates Human 144-247 E. coli 12154080
TRPC5 Link NHERF1 Fusion protein-pull down assay Mouse Full-length In vitro translation Mouse 1-107 E. coli 10980202
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Validation: In vivo validation Toggle
  Assay with endogenous proteins Assay with overexpressed proteins Reference
Cell or tissue Cell or tissue TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Species Region
TRPC4 Link NHERF1 Co-immunofluorescence staining COS-7 Human Full-length Not specifed Full-length 16144838
TRPC4 Link NHERF1 Co-immunoprecipitation HEK293 Mouse Full-length Not used 10980202
TRPC4 Link NHERF1 Co-immunoprecipitation Mouse brain tissue 10980202
TRPC4 Link NHERF1 Co-immunoprecipitation HEK293 Mouse Full-length Not used 18048348
TRPC4 Link NHERF1 Co-immunoprecipitation HEK293 Human Full-length Human Full-length 12154080
TRPC4 Link NHERF1 Co-immunoprecipitation COS-7 Human Full-length Not specifed Full-length 16144838
TRPC4 Link NHERF1 Co-immunoprecipitation Human fetal brain astrocytes 15540229
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Characterization Toggle
  Binding region mapping Stoichiometry Affinity (Kd) Reference
TRP channel Interactor
TRP channel Interactor Method Species Region Species Region
TRPC4 Link NHERF1 Co-immunoprecipitation Human 975-977 (in α varint) Human 130-328 12154080
TRPC4 Link NHERF1 Fusion protein-pull down assay Mouse 688-974 Mouse 1-107 10980202
TRPC4 Link NHERF1 Fusion protein-pull down assay Human 975-977 (in α varint) Human 1-102 12154080
TRPC4 Link NHERF1 Fusion protein-pull down assay Human 975-977 (in α varint) Human 144-247 12154080
TRPC5 Link NHERF1 Fusion protein-pull down assay Mouse 648-975 Mouse 1-107 10980202
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Functional consequence Toggle
TRP channel Interactor Method Post-translational modification Subcellular trafficking Activity Reference
TRPC4 Link NHERF1 Cell surface biotinylation Increase in plasma membrane level 12154080
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
TRP / Interactor

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