NHERF4 / TRP channels

Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF4 (NHERF4)
Official Symbol: PDZD3 provided by HGNC
Function:
Acts as a regulatory protein that associates with GUCY2C and negatively modulates its heat-stable enterotoxin-mediated activation. Stimulates SLC9A3 activity in the presence of elevated calcium ions.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).
Species External DB Questionmark
Entrez Gene UniprotKB DIP IntAct MINT BioGRID STRING IUPHAR-DB KEGG OMIM
Human
Seq
79849External Q86UT5External DIP-41141NExternal   MINT-1773252External 122940External Q86UT5External   hsa:79849External 607146External
Mouse
Seq
170761External Q99MJ6External DIP-41892NExternal   MINT-152467External 228423External Q99MJ6External   mmu:170761External  
Rat
Seq
500986External D4A7C5External       272293External     rno:500986External  
PPI pairs:
Biological Process:
Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/
Screening Toggle
  Experimental screening Non-experimental screening Reference
TRP channel construct Interactor source
TRP channel Interactor Method Species Region Species Organ/tissue Sample type
TRPV4 Link NHERF4 Inference Prediction 16565876
TRPV5 Link NHERF4 Yeast two-hybrid Mouse C-terminus Mouse Kidney cDNA library 16565876
TRPV6 Link NHERF4 Yeast two-hybrid Mouse 575-727 Mouse Kidney cDNA library 17645868
TRPV6 Link NHERF4 Yeast two-hybrid Mouse C-terminus Mouse Kidney cDNA library 16565876
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Validation: In vitro validation Toggle
  Assay with recombinant proteins Reference
TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Expression system Species Region Expression system
TRPV4 Link NHERF4 Fusion protein-pull down assay Mouse C-terminus E. coli Mouse Full-length In vitro translation 16565876
TRPV5 Link NHERF4 Fusion protein-pull down assay Mouse C-terminus E. coli Mouse Full-length In vitro translation 16565876
TRPV6 Link NHERF4 Fusion protein-pull down assay Mouse 575-727 E. coli Mouse Full-length In vitro translation 17645868
TRPV6 Link NHERF4 Fusion protein-pull down assay Mouse C-terminus E. coli Mouse Full-length In vitro translation 16565876
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Validation: In vivo validation Toggle
  Assay with endogenous proteins Assay with overexpressed proteins Reference
Cell or tissue Cell or tissue TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Species Region
TRPV5 Link NHERF4 Co-immunofluorescence staining Xenopus oocyte Mouse Full-length Mouse Full-length 16565876
TRPV5 Link NHERF4 Co-immunofluorescence staining Mouse kidney section 16565876
TRPV5 Link NHERF4 Co-immunoprecipitation HEK293 Mouse Full-length Mouse Full-length 16565876
TRPV6 Link NHERF4 Co-immunoprecipitation HEK293 Mouse Full-length Mouse Full-length 16565876
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Characterization Toggle
  Binding region mapping Stoichiometry Affinity (Kd) Reference
TRP channel Interactor
TRP channel Interactor Method Species Region Species Region
TRPV5 Link NHERF4 Fusion protein-pull down assay Mouse 579-617 Mouse Not determined 16565876
TRPV6 Link NHERF4 Fusion protein-pull down assay Mouse 724-727 Mouse Not determined 17645868
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Functional consequence Toggle
TRP channel Interactor Method Post-translational modification Subcellular trafficking Activity Reference
TRPV6 Link NHERF4 Patch clamp Activation 17645868
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
TRP / Interactor

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