PLCγ1 / TRP channels

Name: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 (PLCγ1)
Official Symbol: PLCG1 provided by HGNC
Function:
Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).
Species External DB Questionmark
Entrez Gene UniprotKB DIP IntAct MINT BioGRID STRING IUPHAR-DB KEGG OMIM
Human
Seq		 5335External P19174External DIP-100NExternal P19174External MINT-102915External 111351External P19174External   hsa:5335External 172420External
Mouse
Seq		 18803External Q62077External DIP-29284NExternal Q62077External MINT-124146External 202238External Q62077External   mmu:18803External  
Rat
Seq		 25738External P10686External DIP-2863NExternal P10686External MINT-231720External 247766External P10686External   rno:25738External  
Biological Process:
Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Screening Toggle
  Experimental screening Non-experimental screening Reference
TRP channel construct Interactor source
TRP channel Interactor Method Species Region Species Organ/tissue Sample type
TRPC1 Link PLCγ1 Inference Prediction 15654973
TRPC2 Link PLCγ1 Inference Prediction 15329338
TRPC3 Link PLCγ1 Inference Prediction 12437926
TRPC4 Link PLCγ1 Inference Prediction 12437926
TRPC6 Link PLCγ1 Inference Prediction 19074769
TRPM6 Link PLCγ1 Inference Prediction 22180838
TRPM7 Link PLCγ1 Inference Prediction 11941371
TRPV1 Link PLCγ1 Inference Prediction 15744307
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
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Validation: In vitro validation Toggle
  Assay with recombinant proteins Reference
TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Expression system Species Region Expression system
TRPC3 Link PLCγ1 Yeast two-hybrid Human 1-171 Yeast Rat 796-920 Yeast 15744307
TRPC3 Link PLCγ1 Yeast two-hybrid Human 1-340 Yeast Rat 750-1290 Yeast 12437926
TRPC4 Link PLCγ1 Yeast two-hybrid Human 1-330 Yeast Rat 750-1290 Yeast 12437926
TRPM6 Link PLCγ1 Fusion protein-pull down assay Not specified Kinase domain E. coli Not specified Full-length HEK293 lysates 22180838
TRPM7 Link PLCγ1 Fusion protein-pull down assay Rat 1352-1862 E. coli Rat Full-length HEK293T lysates 11941371
TRPV1 Link PLCγ1 Yeast two-hybrid Rat 761-835 Yeast Rat 796-920 Yeast 15744307
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Validation: In vivo validation Toggle
  Assay with endogenous proteins Assay with overexpressed proteins Reference
Cell or tissue Cell or tissue TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Species Region
TRPC1 Link PLCγ1 Co-immunofluorescence staining Human keratinocyte 15654973
TRPC1 Link PLCγ1 Co-immunoprecipitation Human keratinocyte 15654973
TRPC2 Link PLCγ1 Co-immunoprecipitation CHO-S Mouse Full-length Rat Full-length 15329338
TRPC2 Link PLCγ1 Co-immunoprecipitation Mouse erythroblasts 15329338
TRPC2 Link PLCγ1 Co-immunoprecipitation CHO-S Mouse Full-length Rat Full-length 22088247
TRPC3 Link PLCγ1 Co-immunoprecipitation HEK293T Human Full-length Rat Full-length 19074769
TRPC3 Link PLCγ1 Co-immunoprecipitation HEK293 Mouse Full-length Human Full-length 19289841
TRPC3 Link PLCγ1 Co-immunoprecipitation HEK293T Human Full-length Rat Full-length 21757714
TRPC3 Link PLCγ1 Co-immunoprecipitation Human erythroid cell 18276585
TRPC3 Link PLCγ1 Co-immunoprecipitation HEK293T Human Full-length Rat Full-length 18276585
TRPC3 Link PLCγ1 Co-immunoprecipitation HEK293T Human Full-length Rat Full-length 12437926
TRPC6 Link PLCγ1 Co-immunoprecipitation HEK293T Human Full-length Rat Full-length 19074769
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Characterization Toggle
  Binding region mapping Stoichiometry Affinity (Kd) Reference
TRP channel Interactor
TRP channel Interactor Method Species Region Species Region
TRPC3 Link PLCγ1 Fusion protein-pull down assay Human 1-52 Rat 794-933 16500902
TRPC3 Link PLCγ1 Fusion protein-pull down assay Human 40-48 Rat 861-920 15744307
TRPC3 Link PLCγ1 Yeast two-hybrid Human 40-46 Rat 861-920 15744307
TRPC3 Link PLCγ1 Yeast two-hybrid Human 1-340 Rat 780-920 12437926
TRPC4 Link PLCγ1 Yeast two-hybrid Human 1-330 Rat 780-920 12437926
TRPV1 Link PLCγ1 Yeast two-hybrid Rat 761-835 Rat 861-920 15744307
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Functional consequence Toggle
TRP channel Interactor Method Post-translational modification Subcellular trafficking Activity Reference
TRPC1 Link PLCγ1 Calcium measurement Activation 15654973
TRPC3 Link PLCγ1 Calcium measurement Activation 15744307
TRPC3 Link PLCγ1 Calcium measurement Activation 12437926
TRPM7 Link PLCγ1 Patch clamp Inhibition 11941371
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
TRP / Interactor

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