SNAPIN / TRP channels

Name: SNARE-associated protein Snapin (SNAPIN)
Official Symbol: SNAPIN provided by HGNC
Function:
Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. Plays a role in intracellular vesicle trafficking. May modulate a step between vesicle priming, fusion and calcium-dependent neurotransmitter release through its ability to potentiate the interaction of synaptotagmin with the SNAREs and the plasma-membrane-associated protein SNAP25. Its phosphorylation state influences exocytotic protein interactions and may regulate synaptic vesicle exocytosis. May also have a role in the mechanisms of SNARE-mediated membrane fusion in non-neuronal cells.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).
Species External DB Questionmark
Entrez Gene UniprotKB DIP IntAct MINT BioGRID STRING IUPHAR-DB KEGG OMIM
Human
Seq
23557External O95295External DIP-31359NExternal O95295External MINT-5002303External 117101External O95295External   hsa:23557External 607007External
Mouse
Seq
20615External Q9Z266External DIP-44321NExternal Q9Z266External MINT-3389684External 203363External Q9Z266External   mmu:20615External  
Rat
Seq
295217External P60192External DIP-37034NExternal P60192External MINT-3389957External 254916External P60192External   rno:295217External  
PPI pairs:
Biological Process:
Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/
Screening Toggle
  Experimental screening Non-experimental screening Reference
TRP channel construct Interactor source
TRP channel Interactor Method Species Region Species Organ/tissue Sample type
TRPC6 Link SNAPIN Inference Prediction 17684020
TRPM7 Link SNAPIN Yeast two-hybrid Human 87-326 Human Brain cDNA library 17088214
TRPV1 Link SNAPIN Yeast two-hybrid Rat 1-414 Rat Brain cDNA library 15066994
TRPV1 Link SNAPIN Yeast two-hybrid Rat 1-432 Human Fetal brain cDNA library 17074976
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Validation: In vitro validation Toggle
  Assay with recombinant proteins Reference
TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Expression system Species Region Expression system
TRPM7 Link SNAPIN Fusion protein-pull down assay Human 83-326 In vitro translation Human Full-length Not specified 17088214
TRPV1 Link SNAPIN Fusion protein-pull down assay Rat 1-414 E. coli Human Full-length In vitro translation 15066994
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Validation: In vivo validation Toggle
  Assay with endogenous proteins Assay with overexpressed proteins Reference
Cell or tissue Cell or tissue TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Species Region
TRPC6 Link SNAPIN Co-immunoprecipitation Rat brain cortex 17684020
TRPM7 Link SNAPIN Co-immunoprecipitation Rat brain synaptic vesicle 17088214
TRPV1 Link SNAPIN Co-immunoprecipitation HEK293 Human Full-length Human Full-length 15066994
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Characterization Toggle
  Binding region mapping Stoichiometry Affinity (Kd) Reference
TRP channel Interactor
TRP channel Interactor Method Species Region Species Region
TRPM7 Link SNAPIN Fusion protein-pull down assay Human 87-326 Human 43-68 17088214
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
TRP / Interactor

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