TRP channels

TRPC
- TRPC1
- TRPC2
- TRPC3
- TRPC4
- TRPC5
- TRPC6
- TRPC7
TRPV
- TRPV1
- TRPV2
- TRPV3
- TRPV4
- TRPV5
- TRPV6
TRPM
- TRPM1
- TRPM2
- TRPM3
- TRPM4
- TRPM5
- TRPM6
- TRPM7
- TRPM8
TRPML
- TRPML1
- TRPML2
- TRPML3
TRPP
- TRPP1
- TRPP2
- TRPP3
TRPA
- TRPA1

» Citation

TRPC3 / interactors

At a glance
In detail

Basic Information
External Database
PPI pairs (117)
Biological Process (6)
Disease (0)

Name: Short transient receptor potential channel 3 (TRPC3)

Official Symbol: TRPC3 provided by HGNC

Function:

Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C, and by inositol 1,4,5-triphosphate receptors (ITPR) with bound IP3. May also be activated by internal calcium store depletion.

Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species

External DB

Entrez Gene

UniprotKB

DIP

IntAct

MINT

BioGRID

STRING

IUPHAR-DB

KEGG

OMIM

Human

Mouse

Rat

PPI pairs:

14-3-3 protein β/α	14-3-3 protein ε	14-3-3 protein η	14-3-3 protein θ	ADT1	ALDOC	AP2A2	AP2B1
AQP-2	ATP synthase β	Aconitase	Annexin 5	BKca	Basp1	CaSR	Calmodulin
Calreticulin	Caskin-1	Cav1.2	Caveolin-1	Citrate synthase	Clathrin-H1	Cytochrome c oxidase 2	Cytochrome c oxidase 5A
DRP-2	Dynamin-1 heavy chain 1	Dynamin-1L	Epo-R	Ezrin	FKBP12	Fibronectin	Fumarase
GC-A	GLUD1	GLUT-4	Gz-α	Gαq	Gαq/11	Homer	Homer-1
IP3R1	IP3R2	IP3R3	Idh3B	Junctate	Junctophilin-2	LETM1	MAP2
MDH1	MDH2	MG29	MxA	Myosin-10	NCAM1	NCX1	NKAα1
NKAα2	NKAα3	Neurofascin	Neuromodulin	ORP-150	Orai1	Orai2	Orai3
PDE3A	PEBP1	PGK1	PKA	PKCβ	PKG1	PLCβ1	PLCβ2
PLCβ3	PLCγ1	PLCγ2	PMCA	PMCA2	PMCA3	PMCA4	Pyruvate kinase M1/M2
RACK1	RAP1A	RAP1B	RNF24	RyR	RyR2	SERCA2	SNAP23
SNAP25	SOD2	STIM1	STIM2	SV2A	Slc1a2	Slc1a3	Src
Synapsin1	Synaptotagmin 1	Syntaxin1a	Syntaxin3	TRPC1	TRPC4	TRPC5	TRPC6
TRPC7	TRPM4	TRPP1	Tenascin-R	TrkB	UCH-L1	VAMP2	VAT2
VDAC1	VDAC3	WNK4	p97/VCP	αSNAP

Biological Process:

GO:0007411 : axon guidance
GO:0007602 : phototransduction
GO:0030168 : platelet activation
GO:0010524 : positive regulation of calcium ion transport into cytosol
GO:0033198 : response to ATP
GO:0051592 : response to calcium ion

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/

Disease: No information in OMIM

Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening

Validation: In vitro validation

Validation: In vivo validation

Characterization

Functional consequence

top

Screening
			Experimental screening					Non-experimental screening	Reference
			TRP channel construct		Interactor source
TRP channel	Interactor	Method	Species	Region	Species	Organ/tissue	Sample type
TRPC3	14-3-3 protein β/α	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	14-3-3 protein ε	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	14-3-3 protein η	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	14-3-3 protein θ	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	Aconitase	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	ADT1	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	ALDOC	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	Annexin 5	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	AP2A2	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	AP2B1	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	AQP-2	Inference						Prediction	17699554
TRPC3	ATP synthase β	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	Basp1	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	BKca	Inference						Prediction	19052171
TRPC3	Calmodulin	Inference						Prediction	11248050
TRPC3	Calreticulin	Inference						Prediction	18215135
TRPC3	Caskin-1	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	CaSR	Inference						Prediction	22778254
TRPC3	Cav1.2	Inference						Prediction	21672930
TRPC3	Caveolin-1	Inference						Prediction	11524429
TRPC3	Citrate synthase	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	Clathrin-H1	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	Cytochrome c oxidase 2	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	Cytochrome c oxidase 5A	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	DRP-2	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	Dynamin-1 heavy chain 1	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	Dynamin-1L	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	Epo-R	Inference						Prediction	18276585
TRPC3	Ezrin	Inference						Prediction	11524429
TRPC3	Fibronectin	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	FKBP12	Inference						Prediction	15199065
TRPC3	Fumarase	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	GC-A	Inference						Prediction	22027011
TRPC3	GLUD1	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	GLUT-4	Inference						Prediction	17156765
TRPC3	Gz-α	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	Gαq	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	Gαq/11	Inference						Prediction	11524429
TRPC3	Homer-1	Inference						Prediction	16887806
TRPC3	Idh3B	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	IP3R1	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	IP3R1	Inference						Prediction	10518223
TRPC3	IP3R2	Inference						Prediction	11290752
TRPC3	IP3R3	Inference						Prediction	10611319
TRPC3	Junctate	Inference						Prediction	15302852
TRPC3	Junctophilin-2	Inference						Prediction	18215135
TRPC3	LETM1	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	MAP2	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	MDH1	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	MDH2	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	MG29	Inference						Prediction	18215135
TRPC3	MxA	Inference						Prediction	15757897
TRPC3	Myosin-10	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	NCAM1	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	NCX1	Inference						Prediction	14736881
TRPC3	Neurofascin	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	Neuromodulin	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	NKAα1	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	NKAα1	Inference						Prediction	17012351
TRPC3	NKAα2	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	NKAα3	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	Orai1	Inference						Prediction	17360584
TRPC3	Orai2	Inference						Prediction	17360584
TRPC3	Orai3	Inference						Prediction	17360584
TRPC3	ORP-150	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	p97/VCP	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	PDE3A	Inference						Prediction	21799177
TRPC3	PEBP1	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	PGK1	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	PKA	Inference						Prediction	21799177
TRPC3	PKCβ	Inference						Prediction	20179100
TRPC3	PKG1	Inference						Prediction	14983059
TRPC3	PLCβ1	Inference						Prediction	11524429
TRPC3	PLCβ2	Inference						Prediction	15623527
TRPC3	PLCβ3	Inference						Prediction	15623527
TRPC3	PLCγ1	Inference						Prediction	12437926
TRPC3	PLCγ2	Inference						Prediction	12970180
TRPC3	PMCA	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	PMCA	Speculation						Prediction	16887806
TRPC3	PMCA2	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	PMCA3	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	PMCA4	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	Pyruvate kinase M1/M2	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	RACK1	Yeast two-hybrid	Not specified	1-341	Human	Embroynic kidney	cDNA library		18755685
TRPC3	RAP1A	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	RAP1B	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	RNF24	Inference						Prediction	17850865
TRPC3	RyR	Inference						Prediction	10983988
TRPC3	RyR2	Inference						Prediction	17012351
TRPC3	SERCA2	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	SERCA2	Inference						Prediction	11524429
TRPC3	Slc1a2	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	Slc1a3	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	SNAP23	Inference						Prediction	15327778
TRPC3	SNAP25	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	SOD2	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	SRC	Inference						Prediction	16407161
TRPC3	STIM1	Speculation						Prediction	22640869
TRPC3	STIM2	Speculation						Prediction	22640869
TRPC3	SV2A	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	Synapsin1	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	Synaptotagmin 1	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	Syntaxin1a	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	Syntaxin3	Inference						Prediction	15327778
TRPC3	Tenascin-R	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	TrkB	Inference						Prediction	10677043
TRPC3	TRPC1	Inference						Prediction	9215637
TRPC3	TRPC4	Inference						Prediction	16537542
TRPC3	TRPC5	Inference						Prediction	12857742
TRPC3	TRPC6	Inference						Prediction	12032305
TRPC3	TRPC7	Inference						Prediction	12032305
TRPC3	TRPM4	Yeast two-hybrid	Not used as a bait		Human	Brain	cDNA library		18262493
TRPC3	TRPP1	Inference						Prediction	19812035
TRPC3	UCH-L1	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	VAMP2	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	VAMP2	Yeast two-hybrid	Human	1-341	Rat	Brain	cDNA library		15327778
TRPC3	VAT2	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	VDAC1	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	VDAC3	Affinity purification-mass spectrometry	Not used as a bait		Rat	Brain	Crude membranes		18205297
TRPC3	WNK4	Inference						Prediction	21670282
TRPC3	αSNAP	Yeast two-hybrid	Human	1-341	Rat	Brain	cDNA library		15327778

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation

Validation: In vivo validation

Characterization

Functional consequence

top

Validation: In vitro validation
			Assay with recombinant proteins						Reference
			TRP channel construct			Interactor construct
TRP channel	Interactor	Method	Species	Region	Expression system	Species	Region	Expression system
TRPC3	BKca	Fusion protein-pull down assay	Not used		Mouse podocyte lysates	Chicken	Full-length	E. coli	19052171
TRPC3	Calmodulin	Fusion protein-pull down assay	Human	1-340	In vitro translation	Not specified	Full-length	E. coli	12601176
TRPC3	Calmodulin	Fusion protein-pull down assay	Human	742-795	E. coli	Rat	Full-length	In vitro translation	11248050
TRPC3	FKBP12	Co-immunoprecipitation	Human	Full-length	Sf9 lysates	Human	Full-length	Sf9 lysates	15199065
TRPC3	Homer-1	Fusion protein-pull down assay	Human	Full-length	HEK293 lysates	Human	Full-length	E. coli	16887806
TRPC3	IP3R3	Fusion protein-pull down assay	Human	676-848	In vitro translation	Human	638-926	E. coli	10611319
TRPC3	Junctate	Fusion protein-pull down assay	Bovine	Full-length	HEK293 lysates	Not specified	1-85	Not specified	15302852
TRPC3	Junctophilin-2	Fusion protein-pull down assay	Not used		Rabbit back and leg fast-twich skeletal muscle lysates	Mouse	Full-length	E. coli	19277847
TRPC3	MxA	Fusion protein-pull down assay	Human	1-201	In vitro translation	Human	572-662	E. coli	15757897
TRPC3	NCX1	Fusion protein-pull down assay	Human	670-848	E. coli	Not used		Rat Cardiomyocyte lysates	17129578
TRPC3	NCX1	Fusion protein-pull down assay	Human	742-848	E. coli	Not used		HEK293 lysates	14736881
TRPC3	Orai1	Fusion protein-pull down assay	Human	1-340	In vitro translation	Human	Full-length	E. coli	17360584
TRPC3	Orai1	Fusion protein-pull down assay	Human	676-848	In vitro translation	Human	Full-length	E. coli	17360584
TRPC3	PKCβ	Fusion protein-pull down assay	Mouse	659-836	E. coli	Not used		DT40 lysates	20179100
TRPC3	PLCγ1	Yeast two-hybrid	Human	1-340	Yeast	Rat	750-1290	Yeast	12437926
TRPC3	PLCγ1	Yeast two-hybrid	Human	1-171	Yeast	Rat	796-920	Yeast	15744307
TRPC3	RNF24	Fusion protein-pull down assay	Not specified	1-201	In vitro translation	Human	98-148	E. coli	17850865
TRPC3	SRC	Fusion protein-pull down assay	Mouse	1-340	In vitro translation	Chicken	Full-length	E. coli	16407161
TRPC3	TRPC1	Fusion protein-pull down assay	Not specified	Full-length	HEK293 lysates	Not specified	1-347	E. coli	15834157
TRPC3	TRPC1	Yeast two-hybrid	Not specified	642-842	Yeast	Not specified	642-842	Yeast	15834157
TRPC3	TRPM4	Yeast two-hybrid	Human	Full-length	Yeast	Human	Full-length	Yeast	18262493

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation

Validation: In vivo validation

Characterization

Functional consequence

top

Validation: In vivo validation
			Assay with endogenous proteins	Assay with overexpressed proteins					Reference
			Cell or tissue	Cell or tissue	TRP channel construct		Interactor construct
TRP channel	Interactor	Method	Cell or tissue	Cell or tissue	Species	Region	Species	Region
TRPC3	ADT1	Co-immunoprecipitation	Rat brain lysates						18205297
TRPC3	AQP-2	Co-immunofluorescence staining	Rat kidney duct section						17699554
TRPC3	AQP-2	Co-immunofluorescence staining	Rat kidney medullary collecting duct						20107112
TRPC3	AQP-2	Co-immunoprecipitation	mIMCD-3						17699554
TRPC3	AQP-2	Co-immunoprecipitation	Rat medulla lysates						17699554
TRPC3	BKca	Co-immunofluorescence staining		HEK293T	Not specified	Full-length	Not specified	Full-length	19052171
TRPC3	BKca	Co-immunoprecipitation	Mouse podocyte lysates						19052171
TRPC3	BKca	Co-immunoprecipitation		HEK293T	Not specified	Full-length	Not specified	Full-length	19052171
TRPC3	Calmodulin	Co-immunoprecipitation	Mouse skeletal myotube lysates						18215135
TRPC3	Calreticulin	Co-immunoprecipitation	Mouse skeletal myotube lysates						18215135
TRPC3	CaSR	Co-immunofluorescence staining	Mouse submandibular gland (SMG) ductal tissue section						22778254
TRPC3	CaSR	Co-immunoprecipitation	Mouse submandibular gland (SMG) ductal cell lysates						22778254
TRPC3	Cav1.2	Co-immunoprecipitation	Chick embryonic heart lysates						21672930
TRPC3	Caveolin-1	Co-immunofluorescence staining		HEK293	Human	Full-length	Not used		11524429
TRPC3	Caveolin-1	Co-immunoprecipitation		HEK293	Human	Full-length	Not used		11524429
TRPC3	Clathrin-H1	Co-immunoprecipitation	Rat brain lysates						18205297
TRPC3	DRP-2	Co-immunoprecipitation	Rat brain lysates						18205297
TRPC3	Dynamin-1 heavy chain 1	Co-immunoprecipitation	Rat brain lysates						18205297
TRPC3	Epo-R	Co-immunofluorescence staining		HEK293T	Human	Full-length	Human	Full-length	19074769
TRPC3	Epo-R	Co-immunoprecipitation		HEK293T	Human	Full-length	Human	Full-length	19074769
TRPC3	Epo-R	Co-immunoprecipitation	TF-1						19074769
TRPC3	Epo-R	Co-immunoprecipitation		HEK293T	Human	Full-length	Human	Full-length	21757714
TRPC3	Epo-R	Co-immunoprecipitation		HEK293T	Human	Full-length	Human	Full-length	18276585
TRPC3	Ezrin	Co-immunoprecipitation		HEK293	Human	Full-length	Not used		11524429
TRPC3	FKBP12	Co-immunoprecipitation	Rat cerebral cortex						15199065
TRPC3	GC-A	Co-immunoprecipitation		HEK293	Not specified	Full-length	Rat	Full-length	22027011
TRPC3	GC-A	Co-immunoprecipitation		Mouse cardiac myocyte lysates	Not used		Rat	Full-length	22027011
TRPC3	GC-A	Fluorescence resonance energy transfer		HEK293	Not specified	Full-length	Rat	Full-length	22027011
TRPC3	GLUT-4	Co-immunoprecipitation	Mouse cardiomyocytes						17156765
TRPC3	Gz-α	Co-immunoprecipitation	Rat brain lysates						18205297
TRPC3	Gαq/11	Co-immunoprecipitation		HEK293	Human	Full-length	Not used		11524429
TRPC3	Gαq/11	Co-immunoprecipitation		MDCK	Not specified	Full-length	Not used		15623527
TRPC3	Homer	Co-immunoprecipitation	Mouse skeletal myotube lysates						18215135
TRPC3	IP3R1	Co-immunoprecipitation	HEK293						20022948
TRPC3	IP3R1	Co-immunoprecipitation		HEK293	Human	Full-length	Not used		11524429
TRPC3	IP3R1	Co-immunoprecipitation	Rat cerebral arterial lysates						20378853
TRPC3	IP3R1	Co-immunoprecipitation	Rat mesenteric artery lysates						23045459
TRPC3	IP3R1	Co-immunoprecipitation	Rat brain lysates						18205297
TRPC3	IP3R1	Co-immunoprecipitation		HEK293	Human	Full-length	Rat	Full-length	10518223
TRPC3	IP3R1	Co-immunoprecipitation		MDCK	Not specified	Full-length	Not used		15623527
TRPC3	IP3R2	Co-immunoprecipitation		HEK293	Human	Full-length	Not used		11524429
TRPC3	IP3R2	Co-immunoprecipitation	Sheep uterine arterial endothelial cell						16899571
TRPC3	IP3R3	Co-immunofluorescence staining		HEK293	Human	Full-length	Not used		11524429
TRPC3	IP3R3	Co-immunofluorescence staining	Mouse pancreatic acini						16887806
TRPC3	IP3R3	Co-immunofluorescence staining	Mouse parotid acini						16887806
TRPC3	IP3R3	Co-immunofluorescence staining	Mouse submandibular gland duct						16887806
TRPC3	IP3R3	Co-immunoprecipitation		HEK293	Human	Full-length	Not used		10983988
TRPC3	IP3R3	Co-immunoprecipitation		HEK293	Human	Full-length	Not used		14505576
TRPC3	IP3R3	Co-immunoprecipitation		HEK293	Human	Full-length	Not used		11524429
TRPC3	IP3R3	Co-immunoprecipitation	Mouse brain tissue						16887806
TRPC3	IP3R3	Co-immunoprecipitation	Mouse pancreatic tissue						16887806
TRPC3	IP3R3	Co-immunoprecipitation	Mouse submandibular gland lysates						16887806
TRPC3	IP3R3	Co-immunoprecipitation		HEK293T	Human	Full-length	Human	Full-length	10611319
TRPC3	IP3R3	Co-immunoprecipitation		MDCK	Not specified	Full-length	Not used		15623527
TRPC3	Junctophilin-2	Co-immunoprecipitation		Mouse skeletal muscle myoblast lysates	Not used		Mouse	Full-length	20095964
TRPC3	Junctophilin-2	Co-immunoprecipitation	Mouse skeletal myotube lysates						18215135
TRPC3	MG29	Co-immunoprecipitation	Mouse skeletal myotube lysates						18215135
TRPC3	MxA	Co-immunoprecipitation		HEK293T	Human	Full-length	Human	Full-length	15757897
TRPC3	NCX1	Co-immunofluorescence staining	dnTRPC3 TG mouse heart myocyte						20351294
TRPC3	NCX1	Co-immunofluorescence staining	Rat myocyte section						17012351
TRPC3	NCX1	Co-immunofluorescence staining	Rat cardiomyocytes						17129578
TRPC3	NCX1	Co-immunoprecipitation		HEK293	Human	Full-length	Not used		14736881
TRPC3	NCX1	Co-immunoprecipitation		Sf9	Mouse	Not specified	Rat	Not specified	17012351
TRPC3	NCX1	Co-immunoprecipitation	Rat ventricle lysates						17012351
TRPC3	NCX1	Co-immunoprecipitation	Rat cardiomyocytes						17129578
TRPC3	Neuromodulin	Co-immunoprecipitation	Rat brain lysates						18205297
TRPC3	NKAα1	Co-immunofluorescence staining	Rat myocyte section						17012351
TRPC3	NKAα1	Co-immunoprecipitation	Rat ventricle lysates						17012351
TRPC3	NKAα1	Co-immunoprecipitation	Rat brain lysates						18205297
TRPC3	Orai1	Co-immunoprecipitation	HEK293						20022948
TRPC3	Orai1	Co-immunoprecipitation		COS-7	Human	Full-length	Human	Full-length	17360584
TRPC3	Orai2	Co-immunoprecipitation	Human platelets						22640869
TRPC3	Orai2	Co-immunoprecipitation		COS-7	Human	Full-length	Human	Full-length	17360584
TRPC3	Orai3	Co-immunoprecipitation	Human platelets						22640869
TRPC3	Orai3	Co-immunoprecipitation		COS-7	Human	Full-length	Human	Full-length	17360584
TRPC3	PDE3A	Co-immunoprecipitation		HEK293	Not specified	Full-length	Not used		21799177
TRPC3	PKA	Co-immunoprecipitation		HEK293	Not specified	Full-length	Not used		21799177
TRPC3	PKCβ	Co-immunoprecipitation	DT40						20179100
TRPC3	PLCβ1	Co-immunoprecipitation		HEK293	Human	Full-length	Not used		11524429
TRPC3	PLCβ1	Co-immunoprecipitation		MDCK	Not specified	Full-length	Not used		15623527
TRPC3	PLCβ2	Co-immunoprecipitation		MDCK	Not specified	Full-length	Not used		15623527
TRPC3	PLCβ3	Co-immunoprecipitation		MDCK	Not specified	Full-length	Not used		15623527
TRPC3	PLCγ1	Co-immunoprecipitation		HEK293T	Human	Full-length	Rat	Full-length	19074769
TRPC3	PLCγ1	Co-immunoprecipitation		HEK293	Mouse	Full-length	Human	Full-length	19289841
TRPC3	PLCγ1	Co-immunoprecipitation		HEK293T	Human	Full-length	Rat	Full-length	21757714
TRPC3	PLCγ1	Co-immunoprecipitation	Human erythroid cell						18276585
TRPC3	PLCγ1	Co-immunoprecipitation		HEK293T	Human	Full-length	Rat	Full-length	18276585
TRPC3	PLCγ1	Co-immunoprecipitation		HEK293T	Human	Full-length	Rat	Full-length	12437926
TRPC3	PLCγ2	Co-immunoprecipitation		HEK293	Mouse	Full-length	Chicken	Full-length	20179100
TRPC3	PLCγ2	Co-immunoprecipitation		HEK293	Mouse	Full-length	Chicken	Full-length	12970180
TRPC3	PLCγ2	Co-immunoprecipitation		HEK293	Mouse	Full-length	Rat	Full-length	19289841
TRPC3	PMCA	Co-immunoprecipitation	Mouse brain tissue						16887806
TRPC3	PMCA	Co-immunoprecipitation	Mouse pancreatic tissue						16887806
TRPC3	PMCA	Co-immunoprecipitation	Rat brain lysates						18205297
TRPC3	RACK1	Co-immunofluorescence staining		HEK293	Not specified	Full-length	Not used		18755685
TRPC3	RACK1	Co-immunoprecipitation		HEK293	Not specified	Full-length	Not used		18755685
TRPC3	RACK1	Co-immunoprecipitation	Rat brain lysates						18755685
TRPC3	RNF24	Co-immunofluorescence staining		HEK293	Not specified	Full-length	Human	Full-length	17850865
TRPC3	RyR	Co-immunoprecipitation		HEK293	Human	Full-length	Not used		10983988
TRPC3	RyR2	Co-immunofluorescence staining	Rat myocyte section						17012351
TRPC3	RyR2	Co-immunoprecipitation	Rat ventricle lysates						17012351
TRPC3	SERCA2	Co-immunoprecipitation		HEK293	Human	Full-length	Not used		11524429
TRPC3	SERCA2	Co-immunoprecipitation	Rat brain lysates						18205297
TRPC3	SNAP23	Co-immunofluorescence staining	Rat hippocampal neuron						15327778
TRPC3	SNAP23	Co-immunofluorescence staining		HEK293	Human	Full-length	Not used		15327778
TRPC3	SNAP23	Co-immunoprecipitation		HEK293	Human	Full-length	Not used		15327778
TRPC3	SRC	Co-immunoprecipitation		COS-7	Mouse	Full-length	Chicken	Full-length	16407161
TRPC3	STIM1	Co-immunoprecipitation	Human platelets						22640869
TRPC3	STIM1	Co-immunoprecipitation		HEK293	Human	Full-length	Human	Full-length	23068106
TRPC3	STIM2	Co-immunoprecipitation	Human platelets						22640869
TRPC3	Synaptotagmin 1	Co-immunoprecipitation	Rat brain lysates						18205297
TRPC3	Syntaxin3	Co-immunofluorescence staining	Rat hippocampal neuron						15327778
TRPC3	Syntaxin3	Co-immunofluorescence staining		HEK293	Human	Full-length	Not used		15327778
TRPC3	Syntaxin3	Co-immunoprecipitation	Rat hippocampal neuron						15327778
TRPC3	Syntaxin3	Co-immunoprecipitation		HEK293	Human	Full-length	Not used		15327778
TRPC3	Syntaxin3	Co-immunoprecipitation		MDCK	Not specified	Full-length	Not used		15623527
TRPC3	TrkB	Co-immunofluorescence staining	Rat brain pontine neuron						10677043
TRPC3	TrkB	Co-immunoprecipitation	Rat brain lysates						10677043
TRPC3	TRPC1	Co-immunoprecipitation	Rat brain microsome						12857742
TRPC3	TRPC1	Co-immunoprecipitation	HSY cell						15834157
TRPC3	TRPC1	Co-immunoprecipitation	Human platelets						19260825
TRPC3	TRPC1	Co-immunoprecipitation	HEK293						15972814
TRPC3	TRPC1	Co-immunoprecipitation	Rat carotid artery smooth muscle cell						19502552
TRPC3	TRPC1	Co-immunoprecipitation	Mouse skeletal myotube lysates						18215135
TRPC3	TRPC1	Co-immunoprecipitation		HEK293T	Human	Full-length	Human	Full-length	9215637
TRPC3	TRPC1	Co-immunoprecipitation	C2C12						21996795
TRPC3	TRPC1	Co-immunoprecipitation		HEK293	Human	Full-length	Human	Full-length	17486119
TRPC3	TRPC4	Co-immunofluorescence staining	Porcine primary aortic endothelial cell						16537542
TRPC3	TRPC4	Co-immunoprecipitation	Rat brain microsome						12857742
TRPC3	TRPC4	Co-immunoprecipitation		HEK293	Human	Full-length	Mouse	Full-length	16537542
TRPC3	TRPC4	Co-immunoprecipitation	Porcine primary aortic endothelial cell						16537542
TRPC3	TRPC4	Co-immunoprecipitation	TRPC3 dnTRPC4 DTG mouse heart lysataes						20351294
TRPC3	TRPC5	Co-immunoprecipitation	Rat brain microsome						12857742
TRPC3	TRPC6	Co-immunofluorescence staining	MDA-MB-231						19689790
TRPC3	TRPC6	Co-immunoprecipitation		MDCK	Not specified	Full-length	Not used		15623527
TRPC3	TRPC6	Co-immunoprecipitation		HEK293T	Human	Full-length	Human	Full-length	19074769
TRPC3	TRPC6	Co-immunoprecipitation	TF-1						19074769
TRPC3	TRPC6	Co-immunoprecipitation	dnTRPC6 Neonatal rat ventricular myocytes						20351294
TRPC3	TRPC6	Co-immunoprecipitation	Human platelets						19260825
TRPC3	TRPC6	Co-immunoprecipitation	MDA-MB-231						19689790
TRPC3	TRPC6	Co-immunoprecipitation		HEK293	Human	Full-length	Human	Full-length	12032305
TRPC3	TRPC6	Co-immunoprecipitation	Rat brain synaptome						12377790
TRPC3	TRPC6	Co-immunoprecipitation		Sf9	Human	Full-length	Mouse	Full-length	12377790
TRPC3	TRPC6	Co-immunoprecipitation	Human platelets						16270640
TRPC3	TRPC6	Fluorescence resonance energy transfer		HEK293	Human	Full-length	Human	Full-length	12032305
TRPC3	TRPC7	Co-immunoprecipitation		Sf9	Human	Full-length	Mouse	Full-length	12377790
TRPC3	TRPC7	Co-immunoprecipitation	Rat brain synaptome						12377790
TRPC3	TRPC7	Co-immunoprecipitation	Rat ventricular myocytes						18502908
TRPC3	TRPC7	Co-immunoprecipitation		CHO	Human	Full-length	Mouse	1-494	15537648
TRPC3	TRPC7	Fluorescence resonance energy transfer		HEK293	Human	Full-length	Mouse	Full-length	12032305
TRPC3	TRPM4	Co-immunofluorescence staining		HEK293T	Human	Full-length	Human	Full-length	18262493
TRPC3	TRPM4	Co-immunoprecipitation		HEK293T	Human	Full-length	Human	Full-length	18262493
TRPC3	TRPP1	Co-immunofluorescence staining		LLC-PK1	Not used		Human	1-697	19812035
TRPC3	TRPP1	Co-immunoprecipitation		HEK293	Mouse	Full-length	Human	1-697	19812035
TRPC3	VAMP2	Co-immunofluorescence staining	Rat hippocampal neuron						15327778
TRPC3	VAMP2	Co-immunofluorescence staining	HSG cell						15327778
TRPC3	VAMP2	Co-immunofluorescence staining		HEK293	Human	Full-length	Not used		15327778
TRPC3	VAMP2	Co-immunoprecipitation	Rat hippocampal neuron						15327778
TRPC3	VAMP2	Co-immunoprecipitation		HEK293	Human	Full-length	Not used		15327778
TRPC3	WNK4	Co-immunoprecipitation	Rat aorta lysates						21670282
TRPC3	WNK4	Co-immunoprecipitation		HEK293T	Human	Full-length	Mouse	Full-length	21670282
TRPC3	αSNAP	Co-immunofluorescence staining	Rat hippocampal neuron						15327778
TRPC3	αSNAP	Co-immunofluorescence staining	HSG cell						15327778
TRPC3	αSNAP	Co-immunofluorescence staining		HEK293	Human	Full-length	Not used		15327778
TRPC3	αSNAP	Co-immunoprecipitation		HEK293	Human	Full-length	Not used		15327778

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation

Validation: In vivo validation

Characterization

Functional consequence

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Characterization
			Binding region mapping				Stoichiometry	Affinity (Kd)	Reference
			TRP channel		Interactor
TRP channel	Interactor	Method	Species	Region	Species	Region
TRPC3	Calmodulin	Fluorescence probe labeling	Human	764-793	Bovine	Not determined		100 nM	11248050
TRPC3	Calmodulin	Fluorescence probe labeling	Human	764-793	Not specified	Not determined		100 nM	11290752
TRPC3	Calmodulin	Fusion protein-pull down assay	Human	761-795	Not specified	Not determined			11290752
TRPC3	Epo-R	Co-immunoprecipitation	Human	671-746	Human	Not determined			19074769
TRPC3	IP3R1	Co-immunoprecipitation	Human	Not determined	Rat	IP3 binding domain			10518223
TRPC3	IP3R1	Fusion protein-pull down assay	Mouse	725-848	Not specified	654-688			11290752
TRPC3	IP3R2	Fusion protein-pull down assay	Mouse	725-848	Not specified	675-695			11290752
TRPC3	IP3R3	Co-immunoprecipitation	Human	775-787	Rat	Not determined			18276585
TRPC3	IP3R3	Fusion protein-pull down assay	Human	761-795	Human	669-698			11248050
TRPC3	IP3R3	Fusion protein-pull down assay	Human	761-795	Human	669-698			11290752
TRPC3	IP3R3	Fusion protein-pull down assay	Mouse	725-848	Human	681-698			11290752
TRPC3	IP3R3	Fusion protein-pull down assay	Human	777-797	Human	669-698			10611319
TRPC3	IP3R3	Fusion protein-pull down assay	Human	777-797	Human	751-821			10611319
TRPC3	Junctophilin-2	Fusion protein-pull down assay	Mouse	Not determined	Mouse	143-234			19277847
TRPC3	MxA	Fusion protein-pull down assay	Human	1-201	Human	572-662			15757897
TRPC3	NCX1	Fusion protein-pull down assay	Human	670-848	Rat	Not determined			17129578
TRPC3	PKCβ	Fusion protein-pull down assay	Mouse	659-836	Chicken	Not determined			20179100
TRPC3	PLCγ1	Fusion protein-pull down assay	Human	1-52	Rat	794-933			16500902
TRPC3	PLCγ1	Fusion protein-pull down assay	Human	40-48	Rat	861-920			15744307
TRPC3	PLCγ1	Yeast two-hybrid	Human	40-46	Rat	861-920			15744307
TRPC3	PLCγ1	Yeast two-hybrid	Human	1-340	Rat	780-920			12437926
TRPC3	PLCγ2	Co-immunoprecipitation	Mouse	Not determined	Chicken	810-829			12970180
TRPC3	RACK1	Yeast two-hybrid	Not specified	221-342	Human	Not determined			18755685
TRPC3	SRC	Co-immunoprecipitation	Mouse	1-370	Chicken	SH2 domain			16407161
TRPC3	TRPC1	Yeast two-hybrid	Not specified	1-341	Not specified	1-73			15834157
TRPC3	TRPM4	Co-immunoprecipitation	Human	Not determined	Human	858-1188			18262493
TRPC3	TRPP1	Fusion protein-pull down assay	Mouse	659-753	Human	1-697			19812035
TRPC3	VAMP2	Yeast two-hybrid	Human	1-123	Rat	Not determined			15327778
TRPC3	WNK4	Co-immunoprecipitation	Human	1-196	Mouse	441-799			21670282
TRPC3	WNK4	Co-immunoprecipitation	Human	1-196	Mouse	441-1032			21670282

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation

Validation: In vivo validation

Characterization

Functional consequence

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Functional consequence
TRP channel	Interactor	Method	Post-translational modification	Subcellular trafficking	Activity	Reference
TRPC3	Calmodulin	Patch clamp			Inhibition	11248050
TRPC3	CaSR	Cell surface biotinylation		Increase in plasma membrane level		22778254
TRPC3	Epo-R	Calcium measurement			Activation	18276585
TRPC3	Epo-R	Patch clamp			Activation	18276585
TRPC3	Homer-1	Cell surface biotinylation		Increase in plasma membrane level		16887806
TRPC3	Homer-1	Patch clamp			Activation	16887806
TRPC3	IP3R1	Calcium measurement			Activation	10518223
TRPC3	IP3R1	Patch clamp			Activation	10518223
TRPC3	IP3R3	Calcium measurement			Activation	10611319
TRPC3	IP3R3	Calcium measurement			Inhibition	10611319
TRPC3	IP3R3	Patch clamp			Activation	11248050
TRPC3	Junctate	Patch clamp			Activation	15302852
TRPC3	Orai1	Patch clamp			Activation	18287061
TRPC3	PKG1	In vitro PTM assay	Phosphorylation (Tyr-11)			14983059
TRPC3	PKG1	In vitro PTM assay	Phosphorylation (Ser-263)			14983059
TRPC3	PLCγ1	Calcium measurement			Activation	15744307
TRPC3	PLCγ1	Calcium measurement			Activation	12437926
TRPC3	PLCγ2	Calcium measurement			Activation	12970180
TRPC3	PLCγ2	Patch clamp			Activation	12970180
TRPC3	RNF24	Cell surface biotinylation		Decrease in plasma membrane level		17850865
TRPC3	RyR	Patch clamp			Activation	10983988
TRPC3	SRC	Calcium measurement			Activation	16407161
TRPC3	SRC	In vivo PTM assay	Phosphorylation (Tyr-226)			16407161
TRPC3	TRPC1	Patch clamp			New channel creation	10882720
TRPC3	TRPM4	Patch clamp			Inhibition	18262493
TRPC3	TRPP1	Calcium measurement			Activation	19812035
TRPC3	WNK4	Cell surface biotinylation		Decrease in plasma membrane level		21670282
TRPC3	WNK4	Ion influx measurement			Inhibition	21670282

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

TRIP Database

TRPC3 / interactors