TRPC5 / interactors

Name: Short transient receptor potential channel 5 (TRPC5)
Official Symbol: TRPC5 provided by HGNC
Function:
Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Has also been shown to be calcium-selective (By similarity). May also be activated by intracellular calcium store depletion.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).
Species External DB Questionmark
Entrez Gene UniprotKB DIP IntAct MINT BioGRID STRING IUPHAR-DB KEGG OMIM
Human
Seq		 7224External Q9UL62External DIP-35696NExternal Q9UL62External MINT-3082029External 113075External Q9UL62External 490External hsa:7224External 300334External
Mouse
Seq		 22067External Q9QX29External DIP-40847NExternal   MINT-144678External 204331External Q9QX29External   mmu:22067External  
Rat
Seq		 140933External Q8VD38External       250867External Q8VD38External   rno:140933External  
Biological Process:
Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Screening Toggle
  Experimental screening Non-experimental screening Reference
TRP channel construct Interactor source
TRP channel Interactor Method Species Region Species Organ/tissue Sample type
TRPC5 Link AP2B1 Affinity purification-mass spectrometry Endogenous protein Rat Brain Cerebral cortex lysates 16025302
TRPC5 Link CaBP1 Inference Prediction 15895247
TRPC5 Link Calmodulin Inference Prediction 11290752
TRPC5 Link CaMKIIβ Inference Prediction 22135323
TRPC5 Link CAND1 Affinity purification-mass spectrometry Endogenous protein Rat Brain Cerebral cortex lysates 16025302
TRPC5 Link Cav1.2 Inference Prediction 21672930
TRPC5 Link Clathrin-H1 Affinity purification-mass spectrometry Endogenous protein Rat Brain Cerebral cortex lysates 16025302
TRPC5 Link D2R Inference Prediction 18261457
TRPC5 Link Drebrin Affinity purification-mass spectrometry Endogenous protein Rat Brain Cerebral cortex lysates 16025302
TRPC5 Link Dynamin-1 Affinity purification-mass spectrometry Endogenous protein Rat Brain Cerebral cortex lysates 16025302
TRPC5 Link EF2 Affinity purification-mass spectrometry Endogenous protein Rat Brain Cerebral cortex lysates 16025302
TRPC5 Link Enkurin Inference Prediction 15385169
TRPC5 Link FKBP52/FKBP4 Inference Prediction 15199065
TRPC5 Link Gαi3 Inference Prediction 22457348
TRPC5 Link Homer-1 Inference Prediction 14505576
TRPC5 Link Homer-3 Inference Prediction 14505576
TRPC5 Link IP3R3 Inference Prediction 11290752
TRPC5 Link Junctate Inference Prediction 16153633
TRPC5 Link MxA Inference Prediction 15757897
TRPC5 Link Myosin-10 Affinity purification-mass spectrometry Endogenous protein Rat Brain Cerebral cortex lysates 16025302
TRPC5 Link Myosin-5a Affinity purification-mass spectrometry Endogenous protein Rat Brain Cerebral cortex lysates 16025302
TRPC5 Link Myosin-HIIA Affinity purification-mass spectrometry Endogenous protein Rat Brain Cerebral cortex lysates 16025302
TRPC5 Link NCS-1 Inference Prediction 16469785
TRPC5 Link Neurabin-1 Affinity purification-mass spectrometry Endogenous protein Rat Brain Cerebral cortex lysates 16025302
TRPC5 Link Neurabin-2 Affinity purification-mass spectrometry Endogenous protein Rat Brain Cerebral cortex lysates 16025302
TRPC5 Link NHERF1 Inference Prediction 10980202
TRPC5 Link NKAα1 Affinity purification-mass spectrometry Endogenous protein Rat Brain Cerebral cortex lysates 16025302
TRPC5 Link NKAα3 Affinity purification-mass spectrometry Endogenous protein Rat Brain Cerebral cortex lysates 16025302
TRPC5 Link OGDH Affinity purification-mass spectrometry Endogenous protein Rat Brain Cerebral cortex lysates 16025302
TRPC5 Link RNF24 Inference Prediction 17850865
TRPC5 Link RyR1 Affinity purification-mass spectrometry Not used as a bait Rabbit Fast-twitch muscle Triad vesicles 18215135
TRPC5 Link SEC31A Affinity purification-mass spectrometry Endogenous protein Rat Brain Cerebral cortex lysates 16025302
TRPC5 Link SESTD1 Inference Prediction 20164195
TRPC5 Link SNAP91 Affinity purification-mass spectrometry Endogenous protein Rat Brain Cerebral cortex lysates 16025302
TRPC5 Link SRA-1 Affinity purification-mass spectrometry Endogenous protein Rat Brain Cerebral cortex lysates 16025302
TRPC5 Link SRC Inference Prediction 16407161
TRPC5 Link Stathmin1 Inference Prediction 12858178
TRPC5 Link Stathmin2 Inference Prediction 12858178
TRPC5 Link Stathmin3 Yeast two-hybrid Mouse 1-333 Rat Brain cDNA library 12858178
TRPC5 Link Stathmin4 Inference Prediction 12858178
TRPC5 Link STIM1 Inference Prediction 16906149
TRPC5 Link TRF1 Affinity purification-mass spectrometry Not used as a bait Human HEK293T Cell lystaes 20811636
TRPC5 Link TRF2 Affinity purification-mass spectrometry Not used as a bait Human HEK293T Cell lystaes 20811636
TRPC5 Link TRPC1 Inference Prediction 11301024
TRPC5 Link TRPC3 Inference Prediction 12857742
TRPC5 Link TRPC4 Inference Prediction 12032305
TRPC5 Link TRPC6 Inference Prediction 12857742
TRPC5 Link TRPC7 Inference Prediction 18356201
TRPC5 Link TUBGCP2 Affinity purification-mass spectrometry Endogenous protein Rat Brain Cerebral cortex lysates 16025302
TRPC5 Link TUBGCP3 Affinity purification-mass spectrometry Endogenous protein Rat Brain Cerebral cortex lysates 16025302
TRPC5 Link UBC Affinity purification-mass spectrometry Not used as a bait Human U2OS osteosarcoma Cell lystaes 21139048
TRPC5 Link α-actinin Speculation Prediction 16025302
TRPC5 Link α-actinin-1 Affinity purification-mass spectrometry Endogenous protein Rat Brain Cerebral cortex lysates 16025302
TRPC5 Link α-actinin-4 Affinity purification-mass spectrometry Endogenous protein Rat Brain Cerebral cortex lysates 16025302
TRPC5 Link α2-actin Affinity purification-mass spectrometry Endogenous protein Rat Brain Cerebral cortex lysates 16025302
TRPC5 Link αII-spectrin Affinity purification-mass spectrometry Endogenous protein Rat Brain Cerebral cortex lysates 16025302
TRPC5 Link βII-spectrin Affinity purification-mass spectrometry Endogenous protein Rat Brain Cerebral cortex lysates 16025302
TRPC5 Link βIII-spectrin Affinity purification-mass spectrometry Endogenous protein Rat Brain Cerebral cortex lysates 16025302
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Validation: In vitro validation Toggle
  Assay with recombinant proteins Reference
TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Expression system Species Region Expression system
TRPC5 Link CaBP1 Fusion protein-pull down assay Mouse Full-length E. coli Human Full-length E. coli 15895247
TRPC5 Link Calmodulin Fusion protein-pull down assay Mouse 1-331 In vitro translation Not specified Not specified Not specified 15987684
TRPC5 Link Calmodulin Fusion protein-pull down assay Mouse 701-807 In vitro translation Not specified Not specified Not specified 15987684
TRPC5 Link Calmodulin Fusion protein-pull down assay Mouse 762-975 In vitro translation Not specified Not specified Not specified 15987684
TRPC5 Link Calmodulin Fusion protein-pull down assay Mouse 1-321 In vitro translation Not specified Full-length E. coli 12601176
TRPC5 Link D2R Fusion protein-pull down assay Human 619-973 E. coli Human 131-151 E. coli 18261457
TRPC5 Link Enkurin Fusion protein-pull down assay Mouse Full-length E. coli Mouse Full-length HEK293 lysates 15385169
TRPC5 Link Enkurin Yeast two-hybrid Mouse N-terminus Yeast Mouse Full-length Yeast 15385169
TRPC5 Link FKBP52/FKBP4 Co-immunoprecipitation Mouse Full-length Sf9 lysates Human Full-length Sf9 lysates 15199065
TRPC5 Link Gαi3 Fusion protein-pull down assay Human Full E. coli Human Full E. coli 22457348
TRPC5 Link Junctate Fusion protein-pull down assay Not specified Full-length HEK293 lysates Mouse Full-length In vitro translation 16153633
TRPC5 Link MxA Fusion protein-pull down assay Human 1-183 In vitro translation Human 572-662 E. coli 15757897
TRPC5 Link NCS-1 Fusion protein-pull down assay Human 619-973 E. coli Human Full-length E. coli 16469785
TRPC5 Link NCS-1 Yeast two-hybrid Human 619-973 Yeast Human Full-length Yeast 16469785
TRPC5 Link NHERF1 Fusion protein-pull down assay Mouse Full-length In vitro translation Mouse 1-107 E. coli 10980202
TRPC5 Link RNF24 Fusion protein-pull down assay Not specified 1-183 In vitro translation Human 98-148 E. coli 17850865
TRPC5 Link SRC Fusion protein-pull down assay Mouse 630-975 In vitro translation Chicken Full-length E. coli 16407161
TRPC5 Link SRC Fusion protein-pull down assay Mouse 1-321 In vitro translation Chicken Full-length E. coli 16407161
TRPC5 Link Stathmin3 Fusion protein-pull down assay Not specified Full-length In vitro translation Not specified Full-length In vitro translation 12858178
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Validation: In vivo validation Toggle
  Assay with endogenous proteins Assay with overexpressed proteins Reference
Cell or tissue Cell or tissue TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Species Region
TRPC5 Link CaMKIIβ Co-immunoprecipitation Whole-brain lysates 22135323
TRPC5 Link CaMKIIβ Co-immunoprecipitation HEK293T Mouse Full-length Rat Full-length 22135323
TRPC5 Link CaMKIIβ Co-immunoprecipitation cortical neurons 22135323
TRPC5 Link Cav1.2 Co-immunoprecipitation Chick embryonic heart lysates 21672930
TRPC5 Link Clathrin-H1 Co-immunoprecipitation Rat brain lysates 16025302
TRPC5 Link Drebrin Co-immunoprecipitation Rat brain lysates 16025302
TRPC5 Link Dynamin-1 Co-immunoprecipitation Rat brain lysates 16025302
TRPC5 Link FKBP52/FKBP4 Co-immunoprecipitation Rat cerebral cortex 15199065
TRPC5 Link Gαi3 Co-immunoprecipitation HEK293 Human Full-length Human Full-length 22457348
TRPC5 Link Gαi3 Co-immunoprecipitation Rat brain extract 22457348
TRPC5 Link Homer-1 Co-immunoprecipitation HEK293 Mouse Full-length Not used 14505576
TRPC5 Link Homer-3 Co-immunoprecipitation HEK293 Mouse Full-length Not used 14505576
TRPC5 Link IP3R3 Co-immunoprecipitation HEK293 Mouse Full-length Not used 14505576
TRPC5 Link NCS-1 Co-immunoprecipitation HEK293 Human Full-length Human Full-length 16469785
TRPC5 Link NCS-1 Co-immunoprecipitation Rat brain membrane lysates 16469785
TRPC5 Link NKAα1 Co-immunoprecipitation Rat brain lysates 16025302
TRPC5 Link SESTD1 Co-immunoprecipitation HEK293-HM1 stable cell line Mouse Full-length Human Full-length 20164195
TRPC5 Link Stathmin1 Co-immunoprecipitation HEK293 Not specified Full-length Not specified Full-length 12858178
TRPC5 Link Stathmin2 Co-immunoprecipitation HEK293 Not specified Full-length Not specified Full-length 12858178
TRPC5 Link Stathmin3 Co-immunoprecipitation HEK293T Not specified Full-length Not specified Full-length 12858178
TRPC5 Link Stathmin4 Co-immunoprecipitation HEK293 Not specified Full-length Not specified Full-length 12858178
TRPC5 Link STIM1 Co-immunoprecipitation HEK293T Rat Full-length Rat Full-length 22201561
TRPC5 Link STIM1 Co-immunoprecipitation HEK293 Mouse Full-length Human Full-length 16906149
TRPC5 Link STIM1 Co-immunoprecipitation HEK293 Mouse Full-length Not used 17486119
TRPC5 Link TRPC1 Co-immunoprecipitation Rat brain microsome 12857742
TRPC5 Link TRPC1 Co-immunoprecipitation Rat brain microsome 11301024
TRPC5 Link TRPC1 Co-immunoprecipitation HEK293 Mouse Full-length Human Full-length 11301024
TRPC5 Link TRPC1 Co-immunoprecipitation Sf9 Mouse Full-length Human Full-length 12377790
TRPC5 Link TRPC1 Co-immunoprecipitation Rat brain synaptome 12377790
TRPC5 Link TRPC1 Co-immunoprecipitation Rabbit coronary artery 18356201
TRPC5 Link TRPC1 Co-immunoprecipitation Rabbit mesenteric artery 18356201
TRPC5 Link TRPC1 Co-immunoprecipitation Rabbit portal vein 18356201
TRPC5 Link TRPC1 Co-immunoprecipitation Human platelets 16270640
TRPC5 Link TRPC1 Co-immunoprecipitation Mouse vascular smooth muscle cell 21968068
TRPC5 Link TRPC1 Fluorescence resonance energy transfer HEK293 Mouse Full-length Human Full-length 12032305
TRPC5 Link TRPC3 Co-immunoprecipitation Rat brain microsome 12857742
TRPC5 Link TRPC4 Co-immunoprecipitation HEK293 Mouse Full-length Mouse Full-length 12032305
TRPC5 Link TRPC4 Co-immunoprecipitation Sf9 Mouse Full-length Bovine Full-length 12377790
TRPC5 Link TRPC4 Co-immunoprecipitation Rat brain synaptome 12377790
TRPC5 Link TRPC4 Co-immunoprecipitation Human platelets 16270640
TRPC5 Link TRPC4 Fluorescence resonance energy transfer HEK293 Mouse Full-length Mouse Full-length 12032305
TRPC5 Link TRPC4 Fluorescence resonance energy transfer HEK293 Mouse Full-length Mouse Full-length 17624425
TRPC5 Link TRPC6 Co-immunoprecipitation Rat brain microsome 12857742
TRPC5 Link TRPC6 Co-immunoprecipitation Rabbit coronary artery 18356201
TRPC5 Link TRPC7 Co-immunoprecipitation Rabbit portal vein 18356201
TRPC5 Link α-actinin Co-immunoprecipitation Rat brain lysates 16025302
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Characterization Toggle
  Binding region mapping Stoichiometry Affinity (Kd) Reference
TRP channel Interactor
TRP channel Interactor Method Species Region Species Region
TRPC5 Link CaBP1 Fusion protein-pull down assay Mouse 1-331 Human Not determined 15895247
TRPC5 Link CaBP1 Fusion protein-pull down assay Mouse 721-817 Human Not determined 15895247
TRPC5 Link CaBP1 Fusion protein-pull down assay Mouse 791-866 Human Not determined 15895247
TRPC5 Link Calmodulin Fluorescence probe labeling Mouse 702-731 Not specified Not determined 220 nM 11290752
TRPC5 Link Calmodulin Fusion protein-pull down assay Mouse 701-733 Not specified Not determined 11290752
TRPC5 Link Calmodulin Fusion protein-pull down assay Mouse 701-733 Bovine Not determined 15987684
TRPC5 Link Calmodulin Fusion protein-pull down assay Mouse 828-854 Bovine Not determined 15987684
TRPC5 Link CaMKIIβ Co-immunoprecipitation Mouse N-terminus Rat Not determined 22135323
TRPC5 Link CaMKIIβ Co-immunoprecipitation Mouse Not determined Rat C-terminus 22135323
TRPC5 Link D2R Yeast two-hybrid Human 719-738 Human 131-151 18261457
TRPC5 Link Gαi3 Co-immunoprecipitation Human Not determined Human Not determined 22457348
TRPC5 Link IP3R3 Fusion protein-pull down assay Mouse 701-733 Human 669-698 11290752
TRPC5 Link MxA Fusion protein-pull down assay Human 1-183 Human 572-662 15757897
TRPC5 Link NCS-1 Fusion protein-pull down assay Human 619-803 Human Not determined 16469785
TRPC5 Link NCS-1 Fusion protein-pull down assay Human 803-973 Human Not determined 16469785
TRPC5 Link NCS-1 Yeast two-hybrid Human 619-803 Human Not determined 16469785
TRPC5 Link NCS-1 Yeast two-hybrid Human 803-973 Human Not determined 16469785
TRPC5 Link NHERF1 Fusion protein-pull down assay Mouse 648-975 Mouse 1-107 10980202
TRPC5 Link SESTD1 Fusion protein-pull down assay Mouse 707-735 Human 193-406 20164195
TRPC5 Link STIM1 Co-immunoprecipitation Rat Not determined Rat 1798-1887 22201561
TRPC5 Link TRPC4 Fluorescence resonance energy transfer Mouse 1-308 Mouse 1-292 17624425
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Functional consequence Toggle
TRP channel Interactor Method Post-translational modification Subcellular trafficking Activity Reference
TRPC5 Link CaBP1 Patch clamp Inhibition 15895247
TRPC5 Link Gαi3 Patch clamp Activation 22457348
TRPC5 Link NCS-1 Calcium measurement Activation 16469785
TRPC5 Link NCS-1 Patch clamp Activation 16469785
TRPC5 Link SESTD1 Calcium measurement Activation 20164195
TRPC5 Link TRPC1 Patch clamp New channel creation 12857742
TRPC5 Link TRPC1 Patch clamp New channel creation 11301024
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
TRP / Interactor

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