TRPML1 / interactors

Name: Mucolipin-1 (TRPML1)
Official Symbol: MCOLN1 provided by HGNC
Function:
Cation channel probably playing a role in the endocytic pathway and in the control of membrane trafficking of proteins and lipids. Could play a major role in Ca(2+) transport regulating lysosomal exocytosis.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).
Species External DB Questionmark
Entrez Gene UniprotKB DIP IntAct MINT BioGRID STRING IUPHAR-DB KEGG OMIM
Human
Seq		 57192External Q9GZU1External   Q9GZU1External MINT-1409368External 121441External Q9GZU1External 501External hsa:57192External 605248External
Mouse
Seq		 94178External Q99J21External       220458External Q99J21External   mmu:94178External  
Rat
Seq		 288371External D3ZRF9External       252540External     rno:288371External  
Biological Process:
Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease:
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Screening Toggle
  Experimental screening Non-experimental screening Reference
TRP channel construct Interactor source
TRP channel Interactor Method Species Region Species Organ/tissue Sample type
TRPML1 Link ALG-2/PDCD6 Affinity purification-mass spectrometry Human 1-66 HeLa lysates 19864416
TRPML1 Link HOP/STI1 Inference Prediction 19117012
TRPML1 Link HSC70 Yeast two-hybrid Human 94-298 Human Brain cDNA library 19117012
TRPML1 Link HSP40/DNAJC14 Yeast two-hybrid Human 94-298 Human Brain cDNA library 19117012
TRPML1 Link HSP90 Inference Prediction 19117012
TRPML1 Link LAPTM4a Yeast two-hybrid Human Full-length Human adult brain cDNA library 21224396
TRPML1 Link LAPTM4b Yeast two-hybrid Human Full-length Human adult brain cDNA library 21224396
TRPML1 Link LAPTM5 Speculation Prediction 21224396
TRPML1 Link PKA Inference Prediction 17988215
TRPML1 Link RFP/TRIM27 Yeast two-hybrid Human Full-length Human Fetal brain cDNA library 16169070
TRPML1 Link SEC13 Affinity purification-mass spectrometry Human 1-66 HeLa lysates 19864416
TRPML1 Link SEC31A Affinity purification-mass spectrometry Human 1-66 HeLa lysates 19864416
TRPML1 Link SLC35E1 Yeast two-hybrid Human Full-length Human Fetal brain cDNA library 16169070
TRPML1 Link TPC1 Inference Prediction 21540176
TRPML1 Link TPC2 Inference Prediction 21540176
TRPML1 Link TRPML2 Inference Prediction 16606612
TRPML1 Link TRPML3 Inference Prediction 16606612
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Validation: In vitro validation Toggle
  Assay with recombinant proteins Reference
TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Expression system Species Region Expression system
TRPML1 Link ALG-2/PDCD6 Fusion protein-pull down assay Human 1-66 E. coli Human Full-length HeLa lysates 19864416
TRPML1 Link HSC70 Fusion protein-pull down assay Human 94-298 In vitro translation Human Full-length In vitro translation 19117012
TRPML1 Link HSP40/DNAJC14 Fusion protein-pull down assay Human 94-298 In vitro translation Human Full-length In vitro translation 19117012
TRPML1 Link LAPTM4a Yeast two-hybrid Human Full-length Yeast Human 1-651 Yeast 21224396
TRPML1 Link LAPTM4b Yeast two-hybrid Human Full-length Yeast Human 79-141 Yeast 21224396
TRPML1 Link LAPTM5 Yeast two-hybrid Human Full-length Yeast Human Not specified Yeast 21224396
TRPML1 Link SEC31A Fusion protein-pull down assay Human 1-66 E. coli Human Full-length HeLa lysates 19864416
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Validation: In vivo validation Toggle
  Assay with endogenous proteins Assay with overexpressed proteins Reference
Cell or tissue Cell or tissue TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Species Region
TRPML1 Link ALG-2/PDCD6 Co-immunofluorescence staining ARPE-19 19864416
TRPML1 Link HOP/STI1 Co-immunoprecipitation CHO Human Full-length Not used 19117012
TRPML1 Link HSC70 Co-immunoprecipitation CHO Human Full-length Human Full-length 19117012
TRPML1 Link HSC70 Co-immunoprecipitation CHO Human Full-length Not used 19117012
TRPML1 Link HSP40/DNAJC14 Co-immunofluorescence staining CHO Human Full-length Human Full-length 19117012
TRPML1 Link HSP40/DNAJC14 Co-immunoprecipitation CHO Human Full-length Human Full-length 19117012
TRPML1 Link HSP40/DNAJC14 Co-immunoprecipitation CHO Human Full-length Not used 19117012
TRPML1 Link HSP90 Co-immunoprecipitation CHO Human Full-length Not used 19117012
TRPML1 Link LAPTM4a Co-immunofluorescence staining ARPE-19 Human Full-length Human Full-length 21224396
TRPML1 Link LAPTM4a Co-immunoprecipitation HeLa Human Full-length Human Full-length 21224396
TRPML1 Link LAPTM4b Co-immunofluorescence staining ARPE-19 Human Full-length Human Full-length 21224396
TRPML1 Link LAPTM4b Co-immunoprecipitation HeLa Human Full-length Human Full-length 21224396
TRPML1 Link LAPTM5 Co-immunofluorescence staining ARPE-19 Human Full-length Human Full-length 21224396
TRPML1 Link LAPTM5 Co-immunoprecipitation HeLa Human Full-length Human Full-length 21224396
TRPML1 Link TPC1 Co-immunofluorescence staining HEK293T Human Full-length Human Full-length 21540176
TRPML1 Link TPC1 Co-immunofluorescence staining HeLa Human Full-length Human Full-length 21540176
TRPML1 Link TPC1 Co-immunofluorescence staining SK-BR-3 Human Full-length Human Full-length 21540176
TRPML1 Link TPC1 Co-immunoprecipitation HEK293T Human Full-length Human Full-length 21540176
TRPML1 Link TPC2 Co-immunofluorescence staining HEK293T Human Full-length Human Full-length 21540176
TRPML1 Link TPC2 Co-immunofluorescence staining HeLa Human Full-length Human Full-length 21540176
TRPML1 Link TPC2 Co-immunofluorescence staining SK-BR-3 Human Full-length Human Full-length 21540176
TRPML1 Link TPC2 Co-immunoprecipitation HEK293T Human Full-length Human Full-length 21540176
TRPML1 Link TRPML2 Co-immunofluorescence staining HEK293 19557826
TRPML1 Link TRPML2 Co-immunofluorescence staining CHO Human Full-length Human Full-length 19885840
TRPML1 Link TRPML2 Co-immunoprecipitation HEK293 Human Full-length (mutation in the pore region) Human Full-length (mutation in the pore region) 20736310
TRPML1 Link TRPML2 Co-immunoprecipitation HEK293 19557826
TRPML1 Link TRPML2 Co-immunoprecipitation CHO Human Full-length Human Full-length 19885840
TRPML1 Link TRPML2 Fluorescence resonance energy transfer HEK293 Human Full-length Human Full-length 16606612
TRPML1 Link TRPML3 Co-immunofluorescence staining HEK293 19557826
TRPML1 Link TRPML3 Co-immunofluorescence staining CHO Human Full-length Human Full-length 19885840
TRPML1 Link TRPML3 Co-immunoprecipitation HEK293 Human Full-length (mutation in the pore region) Human Full-length (mutation in the pore region) 20736310
TRPML1 Link TRPML3 Co-immunoprecipitation HEK293 Not specified Full-length Human Full-length 19522758
TRPML1 Link TRPML3 Co-immunoprecipitation HEK293 19557826
TRPML1 Link TRPML3 Co-immunoprecipitation CHO Human Full-length Human Full-length 19885840
TRPML1 Link TRPML3 Fluorescence resonance energy transfer HEK293 Human Full-length Mouse Full-length 16606612
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Characterization Toggle
  Binding region mapping Stoichiometry Affinity (Kd) Reference
TRP channel Interactor
TRP channel Interactor Method Species Region Species Region
TRPML1 Link ALG-2/PDCD6 Fusion protein-pull down assay Human 37-49 Human Not determined 19864416
TRPML1 Link SEC31A Fusion protein-pull down assay Human 37-49 Human Not determined 19864416
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Functional consequence Toggle
TRP channel Interactor Method Post-translational modification Subcellular trafficking Activity Reference
TRPML1 Link PKA In vitro PTM assay Phosphorylation (Ser-557) 17988215
TRPML1 Link PKA In vivo PTM assay Phosphorylation (Ser-559) 17988215
TRPML1 Link PKA Patch clamp Inhibition 17988215
TRPML1 Link TRPML2 Patch clamp New channel creation 19885840
TRPML1 Link TRPML3 Patch clamp New channel creation 19885840
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
TRP / Interactor

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