TRPV1 / interactors
- At a glance
- In detail
Name: Transient receptor potential cation channel subfamily V member 1
(TRPV1)
Official Symbol: TRPV1
provided by HGNC
Function:
Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accumbens by affecting AMPA receptors endocytosis (By similarity).
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt)
Nucleic Acids Res. 40: D71-D75 (2012).
| Species |
External DB
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM | |
Human
 |
7442 |
Q8NER1 |
MINT-4721979 |
113281 |
Q8NER1 |
507 |
hsa:7442 |
602076 |
||
|
Mouse
|
193034 |
Q704Y3 |
MINT-5185032 |
228724 |
Q704Y3 |
mmu:193034 |
||||
|
Rat
|
83810 |
O35433 |
DIP-46106N |
O35433 |
MINT-5117510 |
249845 |
O35433 |
rno:83810 |
||
PPI pairs:
Biological Process:
- GO:0007166
: cell surface receptor signaling pathway - GO:0007635 : chemosensory behavior
- GO:0050955 : thermoception
Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
World Wide Web URL: http://www.geneontology.org/
Disease:
No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine,
Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/
World Wide Web URL: http://omim.org/
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
Screening
|
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|---|---|---|---|---|---|---|---|---|---|---|
| Experimental screening | Non-experimental screening | Reference | ||||||||
| TRP channel construct | Interactor source | |||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | |||
| TRPV1 |
|
80K-H | Speculation | Prediction | 19801576 | |||||
| TRPV1 |
|
AKAP-5 | Inference | Prediction | 18381233 | |||||
| TRPV1 |
|
Akt | Inference | Prediction | 21493704 | |||||
| TRPV1 |
|
AMPKα2 | Inference | Prediction | 22451268 | |||||
| TRPV1 |
|
Calmodulin | Inference | Prediction | 12808128 | |||||
| TRPV1 |
|
CaMKII | Inference | Prediction | 21493704 | |||||
| TRPV1 |
|
Catenin-α2 | Yeast two-hybrid | Rat | 1-432 | Human | Fetal brain | cDNA library | 17074976 | |
| TRPV1 |
|
Catenin-δ2 | Yeast two-hybrid | Rat | 1-432 | Human | Fetal brain | cDNA library | 17074976 | |
| TRPV1 |
|
CB1 | Inference | Prediction | 23142606 | |||||
| TRPV1 |
|
Cbl | Inference | Prediction | 19155296 | |||||
| TRPV1 |
|
CDK12 | Yeast two-hybrid | Rat | 1-432 | Human | Fetal brain | cDNA library | 17074976 | |
| TRPV1 |
|
CDK5 | Inference | Prediction | 17194758 | |||||
| TRPV1 |
|
CHERP | Yeast two-hybrid | Rat | 1-432 | Human | Fetal brain | cDNA library | 17074976 | |
| TRPV1 |
|
Contactin-1 | Yeast two-hybrid | Rat | 1-432 | Human | Fetal brain | cDNA library | 17074976 | |
| TRPV1 |
|
DAP-150 | Yeast two-hybrid | Rat | 1-432 | Human | Fetal brain | cDNA library | 17074976 | |
| TRPV1 |
|
DAZAP2 | Yeast two-hybrid | Rat | 1-432 | Human | Fetal brain | cDNA library | 17074976 | |
| TRPV1 |
|
Eferin/Rab11-FIP3 | Yeast two-hybrid | Rat | 684-820 | Mouse | Brain | cDNA library | 15883036 | |
| TRPV1 |
|
EGFR | Inference | Prediction | 19155296 | |||||
| TRPV1 |
|
eNOS | Inference | Prediction | 21493704 | |||||
| TRPV1 |
|
FAF1 | Yeast two-hybrid | Rat | 243-440 | Rat | Sensory neuron | cDNA expression library | 16510717 | |
| TRPV1 |
|
GABARAP | Yeast two-hybrid | Rat | 1-414 | Rat | Brain | cDNA library | 20179142 | |
| TRPV1 |
|
GCN5 | Yeast two-hybrid | Rat | 1-432 | Human | Fetal brain | cDNA library | 17074976 | |
| TRPV1 |
|
HAX-1 | Yeast two-hybrid | Rat | 1-432 | Human | Fetal brain | cDNA library | 17074976 | |
| TRPV1 |
|
ITM2C | Yeast two-hybrid | Rat | 1-432 | Human | Fetal brain | cDNA library | 17074976 | |
| TRPV1 |
|
JMJD2C | Yeast two-hybrid | Rat | 1-432 | Human | Fetal brain | cDNA library | 17074976 | |
| TRPV1 |
|
KCNQ2 | Inference | Prediction | 21844219 | |||||
| TRPV1 |
|
KCNQ3 | Inference | Prediction | 21844219 | |||||
| TRPV1 |
|
KIF2A | Yeast two-hybrid | Rat | 1-432 | Human | Fetal brain | cDNA library | 17074976 | |
| TRPV1 |
|
KIF3B | Yeast two-hybrid | Rat | 1-432 | Human | Fetal brain | cDNA library | 17074976 | |
| TRPV1 |
|
KPNβ1 | Yeast two-hybrid | Rat | 1-432 | Human | Fetal brain | cDNA library | 17074976 | |
| TRPV1 |
|
Neuronatin | Yeast two-hybrid | Rat | 1-432 | Human | Fetal brain | cDNA library | 17074976 | |
| TRPV1 |
|
NKX2.2 | Yeast two-hybrid | Rat | 1-432 | Human | Fetal brain | cDNA library | 17074976 | |
| TRPV1 |
|
NMDAR1 | Inference | Prediction | 22609428 | |||||
| TRPV1 |
|
NMDAR2B | Inference | Prediction | 22609428 | |||||
| TRPV1 |
|
NOMO1 | Yeast two-hybrid | Rat | 1-432 | Human | Fetal brain | cDNA library | 17074976 | |
| TRPV1 |
|
OS-9 | Inference | Prediction | 17932042 | |||||
| TRPV1 |
|
P2RX3 | Inference | Prediction | 19223122 | |||||
| TRPV1 |
|
PACS-1 | Inference | Prediction | 23159249 | |||||
| TRPV1 |
|
PEX10 | Yeast two-hybrid | Rat | 1-432 | Human | Fetal brain | cDNA library | 17074976 | |
| TRPV1 |
|
PI3K-p85α | Inference | Prediction | 17074976 | |||||
| TRPV1 |
|
PI3K-p85β | Yeast two-hybrid | Rat | 1-432 | Human | Fetal brain | cDNA library | 17074976 | |
| TRPV1 |
|
PIRT | Inference | Prediction | 18455988 | |||||
| TRPV1 |
|
PKA | Inference | Prediction | 12194871 | |||||
| TRPV1 |
|
PKARIIα | Inference | Prediction | 18463244 | |||||
| TRPV1 |
|
PKC | Inference | Prediction | 14523239 | |||||
| TRPV1 |
|
PKCε | Inference | Prediction | 11884385 | |||||
| TRPV1 |
|
PLAGL1 | Yeast two-hybrid | Rat | 1-432 | Human | Fetal brain | cDNA library | 17074976 | |
| TRPV1 |
|
PLCγ1 | Inference | Prediction | 15744307 | |||||
| TRPV1 |
|
POLR2G | Yeast two-hybrid | Rat | 1-432 | Human | Fetal brain | cDNA library | 17074976 | |
| TRPV1 |
|
POLR3D | Yeast two-hybrid | Rat | 1-432 | Human | Fetal brain | cDNA library | 17074976 | |
| TRPV1 |
|
PRKD1/PKCμ | Inference | Prediction | 15471852 | |||||
| TRPV1 |
|
RAB26 | Yeast two-hybrid | Rat | 1-432 | Human | Fetal brain | cDNA library | 17074976 | |
| TRPV1 |
|
RANBP9 | Yeast two-hybrid | Rat | 1-432 | Human | Fetal brain | cDNA library | 17074976 | |
| TRPV1 |
|
RPL12 | Yeast two-hybrid | Rat | 1-432 | Human | Fetal brain | cDNA library | 17074976 | |
| TRPV1 |
|
RyR1 | Affinity purification-mass spectrometry | Not used as a bait | Rabbit | Fast-twitch muscle | Triad vesicles | 18215135 | ||
| TRPV1 |
|
SGT | Yeast two-hybrid | Rat | 1-432 | Human | Fetal brain | cDNA library | 17074976 | |
| TRPV1 |
|
SMA3 | Yeast two-hybrid | Rat | 1-432 | Human | Fetal brain | cDNA library | 17074976 | |
| TRPV1 |
|
SNAPIN | Yeast two-hybrid | Rat | 1-414 | Rat | Brain | cDNA library | 15066994 | |
| TRPV1 |
|
SNAPIN | Yeast two-hybrid | Rat | 1-432 | Human | Fetal brain | cDNA library | 17074976 | |
| TRPV1 |
|
SRC | Inference | Prediction | 15084474 | |||||
| TRPV1 |
|
Synaptotagmin 1 | Inference | Prediction | 15066994 | |||||
| TRPV1 |
|
Synaptotagmin 4 | Yeast two-hybrid | Rat | 1-414 | Rat | Brain | cDNA library | 15066994 | |
| TRPV1 |
|
TAK1 | Inference | Prediction | 23142606 | |||||
| TRPV1 |
|
TGS1 | Yeast two-hybrid | Rat | 1-432 | Human | Fetal brain | cDNA library | 17074976 | |
| TRPV1 |
|
Thrombospondin-3 | Yeast two-hybrid | Rat | 1-432 | Human | Fetal brain | cDNA library | 17074976 | |
| TRPV1 |
|
TMEM115 | Yeast two-hybrid | Rat | 1-432 | Human | Fetal brain | cDNA library | 17074976 | |
| TRPV1 |
|
TrkA | Inference | Prediction | 11418861 | |||||
| TRPV1 |
|
TRPA1 | Inference | Prediction | 20231274 | |||||
| TRPV1 |
|
TRPV2 | Inference | Prediction | 15713749 | |||||
| TRPV1 |
|
TRPV3 | Inference | Prediction | 12077606 | |||||
| TRPV1 |
|
UBP5 | Yeast two-hybrid | Rat | 1-432 | Human | Fetal brain | cDNA library | 17074976 | |
| TRPV1 |
|
ZBTB17 | Yeast two-hybrid | Rat | 1-432 | Human | Fetal brain | cDNA library | 17074976 | |
| TRPV1 |
|
α-tubulin-1A | Inference | Prediction | 15569253 | |||||
| TRPV1 |
|
α-tubulin-1B | Inference | Prediction | 22262838 | |||||
| TRPV1 |
|
β-arrestin 2 | Speculation | Prediction | 22952227 | |||||
| TRPV1 |
|
β-tubulin-3 | Inference | Prediction | 17298389 | |||||
| TRPV1 |
|
β-tubulin-5 | Affinity purification-mass spectrometry | Rat | 681-838 | Rat | Spinal cord | Extract | 15569253 | |
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Validation: In vitro validation
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Assay with recombinant proteins | Reference | |||||||||
| TRP channel construct | Interactor construct | |||||||||
| TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | ||
| TRPV1 |
|
AKAP-5 | Fusion protein-pull down assay | Human | 681-839 | E. coli | Not used | HEK293 lysates | 18701070 | |
| TRPV1 |
|
Calmodulin | Fast performance liquid chromatography | Rat | 712?838 | E. coli | Mouse | Full-length | E. coli | 23119017 |
| TRPV1 |
|
Calmodulin | Fusion protein-pull down assay | Rat | C-terminus | E. coli | Bovine | Full-length | Not specified | 12808128 |
| TRPV1 |
|
Calmodulin | Fusion protein-pull down assay | Rat | N-terminus | E. coli | Not specified | Full-length | In vitro translation | 14699077 |
| TRPV1 |
|
Cbl | Fusion protein-pull down assay | Not specified | 680-838 | HEK293 lysates | Not used | A431 Human epithelial carcinoma cell line | 19155296 | |
| TRPV1 |
|
Eferin/Rab11-FIP3 | Fusion protein-pull down assay | Rat | Full-length | HEK293T lysates | Mouse | Full-length | E. coli | 15883036 |
| TRPV1 |
|
EGFR | Fusion protein-pull down assay | Not specified | 680-838 | HEK293 lysates | Not used | A431 Human epithelial carcinoma cell line | 19155296 | |
| TRPV1 |
|
FAF1 | Fusion protein-pull down assay | Rat | 1-440 | E. coli | Rat | Full-length | E. coli | 16510717 |
| TRPV1 |
|
GABARAP | Fusion protein-pull down assay | Not used | Rat brain lysates | Human | Full-length | E. coli | 20179142 | |
| TRPV1 |
|
PI3K-p85α | Fusion protein-pull down assay | Rat | Full-length | HEK293 lysates | Not specified | Full-length | E. coli | 17074976 |
| TRPV1 |
|
PLCγ1 | Yeast two-hybrid | Rat | 761-835 | Yeast | Rat | 796-920 | Yeast | 15744307 |
| TRPV1 |
|
PRKD1/PKCμ | Fusion protein-pull down assay | Rat | Full-length | E. coli | Not specified | Full-length | Not specified | 15471852 |
| TRPV1 |
|
SNAPIN | Fusion protein-pull down assay | Rat | 1-414 | E. coli | Human | Full-length | In vitro translation | 15066994 |
| TRPV1 |
|
Synaptotagmin 1 | Fusion protein-pull down assay | Rat | 1-414 | E. coli | Human | Full-length | In vitro translation | 15066994 |
| TRPV1 |
|
Synaptotagmin 4 | Fusion protein-pull down assay | Rat | 1-414 | E. coli | Human | Full-length | In vitro translation | 15066994 |
| TRPV1 |
|
α-tubulin-1A | Fusion protein-pull down assay | Rat | 681-838 | E. coli | Rat | Full-length | Rat spinal cord extract | 15569253 |
| TRPV1 |
|
β-tubulin-3 | Fusion protein-pull down assay | Rat | 681-838 | E. coli | Not used | Pig brain tissue lysates | 17298389 | |
| TRPV1 |
|
β-tubulin-5 | Fusion protein-pull down assay | Rat | 681-838 | E. coli | Rat | Full-length | Rat spinal cord extract | 15569253 |
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Validation: In vivo validation
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference | ||||||||
| Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | |||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
| TRPV1 |
|
80K-H | Co-immunoprecipitation | HEK293T | Human | Full-length | Human | Full-length | 19801576 | |
| TRPV1 |
|
AKAP-5 | Co-immunofluorescence staining | Rat trigeminal ganglia neuron | 18381233 | |||||
| TRPV1 |
|
AKAP-5 | Co-immunofluorescence staining | Mouse dorsal root ganglion tissue | 18463244 | |||||
| TRPV1 |
|
AKAP-5 | Co-immunofluorescence staining | Rat dorsal root ganglion neuron | 18701070 | |||||
| TRPV1 |
|
AKAP-5 | Co-immunoprecipitation | Rat trigeminal ganglia neuron | 22789851 | |||||
| TRPV1 |
|
AKAP-5 | Co-immunoprecipitation | Rat trigeminal ganglia neuron | 18381233 | |||||
| TRPV1 |
|
AKAP-5 | Co-immunoprecipitation | CHO | Rat | Full-length | Rat | Full-length | 18381233 | |
| TRPV1 |
|
AKAP-5 | Co-immunoprecipitation | Mouse spinalcord tissue | 18463244 | |||||
| TRPV1 |
|
AKAP-5 | Co-immunoprecipitation | Mouse dorsal root ganglion tissue | 18463244 | |||||
| TRPV1 |
|
AKAP-5 | Co-immunoprecipitation | HEK293 | Human | Full-length | Human | Full-length | 23264624 | |
| TRPV1 |
|
AKAP-5 | Co-immunoprecipitation | HEK293 | Human | Full-length | Mouse | Full-length | 23264624 | |
| TRPV1 |
|
AKAP-5 | Co-immunoprecipitation | Rat dorsal root ganglion neuron | 18701070 | |||||
| TRPV1 |
|
AKAP-5 | Co-immunoprecipitation | HEK293 | Human | Full-length | Not used | 18701070 | ||
| TRPV1 |
|
Akt | Co-immunoprecipitation | Bovine aortic endothelial cells | 21493704 | |||||
| TRPV1 |
|
AMPKα2 | Co-immunoprecipitation | Bovine aortic endothelial cells | 22451268 | |||||
| TRPV1 |
|
Calmodulin | Co-immunoprecipitation | HEK293 | Rat | Full-length | Rat | Full-length | 12808128 | |
| TRPV1 |
|
CaMKII | Co-immunoprecipitation | Bovine aortic endothelial cells | 21493704 | |||||
| TRPV1 |
|
CB1 | Co-immunofluorescence staining | Mouse corneal epithelium section | 23142606 | |||||
| TRPV1 |
|
CB1 | Co-immunofluorescence staining | Human corneal epithelial cells | 23142606 | |||||
| TRPV1 |
|
CB1 | Co-immunoprecipitation | Human corneal epithelial cells | 23142606 | |||||
| TRPV1 |
|
Eferin/Rab11-FIP3 | Co-immunofluorescence staining | HEK293T | Rat | Full-length | Human | Full-length | 15883036 | |
| TRPV1 |
|
Eferin/Rab11-FIP3 | Co-immunoprecipitation | HEK293T | Rat | Full-length | Human | Full-length | 15883036 | |
| TRPV1 |
|
EGFR | Co-immunoprecipitation | HEK293 | Not specified | Full-length | Not used | 19155296 | ||
| TRPV1 |
|
EGFR | Co-immunoprecipitation | HaCaT | 19155296 | |||||
| TRPV1 |
|
eNOS | Co-immunoprecipitation | Bovine aortic endothelial cells | 21493704 | |||||
| TRPV1 |
|
eNOS | Co-immunoprecipitation | Bovine aortic endothelial cells | 22451268 | |||||
| TRPV1 |
|
eNOS | Mammalian two-hybrid | Bovine aortic endothelial cells | Rat | Full-length | Human | Full-length | 22451268 | |
| TRPV1 |
|
FAF1 | Co-immunofluorescence staining | Rat dorsal root ganglion cell | 16510717 | |||||
| TRPV1 |
|
FAF1 | Co-immunoprecipitation | Rat dorsal root ganglion cell | 16510717 | |||||
| TRPV1 |
|
FAF1 | Co-immunoprecipitation | HEK293T | Rat | Full-length | Rat | Full-length | 16510717 | |
| TRPV1 |
|
GABARAP | Co-immunofluorescence staining | Neonatal rat dorsal root ganglion | 20179142 | |||||
| TRPV1 |
|
GABARAP | Fluorescence resonance energy transfer | HEK293 | Rat | Full-length | Human | Full-length | 20179142 | |
| TRPV1 |
|
KCNQ2 | Co-immunoprecipitation | HEK293 | Human | Full-length | Human | Full-length | 21844219 | |
| TRPV1 |
|
KCNQ3 | Co-immunoprecipitation | HEK293 | Human | Full-length | Human | Full-length | 21844219 | |
| TRPV1 |
|
NMDAR1 | Co-immunofluorescence staining | Rat trigeminal ganglia lysates | 22609428 | |||||
| TRPV1 |
|
NMDAR1 | Co-immunoprecipitation | Rat trigeminal ganglia lysates | 22609428 | |||||
| TRPV1 |
|
NMDAR2B | Co-immunoprecipitation | Rat trigeminal ganglia lysates | 22609428 | |||||
| TRPV1 |
|
OS-9 | Co-immunoprecipitation | HEK293T | Mouse | Full-length | Mouse | Full-length | 17932042 | |
| TRPV1 |
|
P2RX3 | Co-immunoprecipitation | HEK293 | Rat | Full-length | Human | Full-length | 19223122 | |
| TRPV1 |
|
PACS-1 | Co-immunoprecipitation | HEK293 | Human | Full-length | Human | Full-length | 23159249 | |
| TRPV1 |
|
PI3K-p85β | Co-immunoprecipitation | HEK293 | Rat | Full-length | Not used | 17074976 | ||
| TRPV1 |
|
PI3K-p85β | Co-immunoprecipitation | Mouse dorsal root ganglion cell | 17074976 | |||||
| TRPV1 |
|
PIRT | Co-immunoprecipitation | HEK293 | Rat | Full-length | Mouse | Full-length | 18455988 | |
| TRPV1 |
|
PKARIIα | Co-immunoprecipitation | Mouse spinalcord tissue | 18463244 | |||||
| TRPV1 |
|
PRKD1/PKCμ | Co-immunofluorescence staining | Rat dorsal root ganglion section | 18063480 | |||||
| TRPV1 |
|
PRKD1/PKCμ | Co-immunoprecipitation | Rat dorsal root ganglion lysates | 18063480 | |||||
| TRPV1 |
|
PRKD1/PKCμ | Co-immunoprecipitation | CHO | Rat | Full-length | Human | Full-length | 15471852 | |
| TRPV1 |
|
PRKD1/PKCμ | Co-immunoprecipitation | Rat dorsal root ganglion neuron | 15471852 | |||||
| TRPV1 |
|
SNAPIN | Co-immunoprecipitation | HEK293 | Human | Full-length | Human | Full-length | 15066994 | |
| TRPV1 |
|
SRC | Co-immunoprecipitation | Rat dorsal root ganglion neuron | 15084474 | |||||
| TRPV1 |
|
SRC | Co-immunoprecipitation | HEK293 | 16319926 | |||||
| TRPV1 |
|
Synaptotagmin 1 | Co-immunoprecipitation | HEK293 | Human | Full-length | Human | Full-length | 15066994 | |
| TRPV1 |
|
Synaptotagmin 4 | Co-immunofluorescence staining | Rat dorsal root ganglion neuron | 15066994 | |||||
| TRPV1 |
|
Synaptotagmin 4 | Co-immunoprecipitation | HEK293 | Human | Full-length | Human | Full-length | 15066994 | |
| TRPV1 |
|
TAK1 | Co-immunoprecipitation | Human corneal epithelial cells | 23142606 | |||||
| TRPV1 |
|
TrkA | Co-immunoprecipitation | HEK293 | Rat | Full-length | Rat | Full-length | 11418861 | |
| TRPV1 |
|
TRPA1 | Co-immunoprecipitation | CHO | Rat | Full-length | Mouse | Full-length | 20231274 | |
| TRPV1 |
|
TRPA1 | Co-immunoprecipitation | Mouse trigeminal ganglia lysates | 20231274 | |||||
| TRPV1 |
|
TRPV2 | Co-immunofluorescence staining | Rat dorsal root ganglion tissue | 16115206 | |||||
| TRPV1 |
|
TRPV2 | Co-immunofluorescence staining | HEK293 | Rat | Full-length | Rat | Full-length | 16237318 | |
| TRPV1 |
|
TRPV2 | Co-immunofluorescence staining | Rat dorsal root ganglion section | 16237318 | |||||
| TRPV1 |
|
TRPV2 | Co-immunoprecipitation | HEK293 | Rat | Full-length | Mouse | Full-length | 15713749 | |
| TRPV1 |
|
TRPV2 | Co-immunoprecipitation | HEK293 | Rat | Full-length | Rat | Full-length | 16115206 | |
| TRPV1 |
|
TRPV2 | Co-immunoprecipitation | Rat brain cortical lysates | 16115206 | |||||
| TRPV1 |
|
TRPV2 | Co-immunoprecipitation | HEK293 | Human | Full-length | Human | Full-length | 16237318 | |
| TRPV1 |
|
TRPV2 | Co-immunoprecipitation | Rat dorsal root ganglion derived cell line F11 | Rat | Full-length | Rat | Full-length | 16237318 | |
| TRPV1 |
|
TRPV2 | Co-immunoprecipitation | Rat dorsal root ganglion lysates | 16237318 | |||||
| TRPV1 |
|
TRPV2 | Co-immunoprecipitation | Rat spinal cord lysates | 16237318 | |||||
| TRPV1 |
|
TRPV3 | Co-immunoprecipitation | HEK293 | Human | Full-length | Human | Full-length | 12077606 | |
| TRPV1 |
|
α-tubulin-1B | Fluorescence resonance energy transfer | CHO | Mouse | Full-length | Human | Full-length | 22262838 | |
| TRPV1 |
|
β-arrestin 2 | Co-immunoprecipitation | Rat trigeminal ganglia sensory neuron | 22952227 | |||||
| TRPV1 |
|
β-tubulin-5 | Co-immunoprecipitation | Rat dorsal root ganglion derived cell line F11 | Rat | Full-length | Not used | 15569253 | ||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Characterization
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | |||||||
| TRP channel | Interactor | |||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
| TRPV1 |
|
AKAP-5 | Co-immunoprecipitation | Human | 736-749 | Human | Not determined | 18701070 | ||
| TRPV1 |
|
AKAP-5 | Fusion protein-pull down assay | Human | 736-749 | Human | Not determined | 18701070 | ||
| TRPV1 |
|
Calmodulin | Fast performance liquid chromatography | Rat | 712?838 | Mouse | Not determined | 23119017 | ||
| TRPV1 |
|
Calmodulin | Fusion protein-pull down assay | Rat | 767-801 | Bovine | Not determined | 12808128 | ||
| TRPV1 |
|
Calmodulin | Fusion protein-pull down assay | Rat | ARD domain | Not specified | Not determined | 20926660 | ||
| TRPV1 |
|
Calmodulin | Fusion protein-pull down assay | Rat | 189-222 | Not specified | Not determined | 14699077 | ||
| TRPV1 |
|
Calmodulin | Fusion protein-pull down assay | Human | 101-364 | Not specified | Not determined | 19864432 | ||
| TRPV1 |
|
Eferin/Rab11-FIP3 | Yeast two-hybrid | Rat | 684-820 | Mouse | 345-444 | 15883036 | ||
| TRPV1 |
|
FAF1 | Fusion protein-pull down assay | Rat | 243-440 | Rat | 110-169 | 16510717 | ||
| TRPV1 |
|
GABARAP | Fusion protein-pull down assay | Rat | 1-414 | Human | 1-36 | 20179142 | ||
| TRPV1 |
|
KCNQ2 | Co-immunoprecipitation | Human | Not determined | Human | 97-318 | 21844219 | ||
| TRPV1 |
|
KCNQ2 | Co-immunoprecipitation | Human | 1-684 | Human | Not determined | 21844219 | ||
| TRPV1 |
|
PI3K-p85α | Fusion protein-pull down assay | Rat | N-terminus | Not specified | SH2 domain | 17074976 | ||
| TRPV1 |
|
PIRT | Co-immunoprecipitation | Rat | Not determined | Mouse | 110-135 | 18455988 | ||
| TRPV1 |
|
PLCγ1 | Yeast two-hybrid | Rat | 761-835 | Rat | 861-920 | 15744307 | ||
| TRPV1 |
|
PRKD1/PKCμ | Fusion protein-pull down assay | Rat | 99-118 | Human | Not determined | 15471852 | ||
| TRPV1 |
|
SRC | Fusion protein-pull down assay | Human | 1-433 | Rat | 83-144 | 16319926 | ||
| TRPV1 |
|
SRC | Fusion protein-pull down assay | Human | 681-839 | Rat | 83-144 | 16319926 | ||
| TRPV1 |
|
Synaptotagmin 4 | Fusion protein-pull down assay | Rat | 1-414 | Human | C2A domain | 15066994 | ||
| TRPV1 |
|
β-tubulin-3 | Fusion protein-pull down assay | Rat | 681-730 | Pig | Not determined | 17298389 | ||
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:
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|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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top
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| Functional consequence
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|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | ||||||
| TRPV1 |
|
AKAP-5 | Cell surface biotinylation | Increase in plasma membrane level | 18701070 | |||||||
| TRPV1 |
|
AKAP-5 | In vivo PTM assay | Phosphorylation | 18381233 | |||||||
| TRPV1 |
|
AKAP-5 | Patch clamp | Activation | 18701070 | |||||||
| TRPV1 |
|
Calmodulin | Patch clamp | Inhibition | 12808128 | |||||||
| TRPV1 |
|
Calmodulin | Patch clamp | Inhibition | 14699077 | |||||||
| TRPV1 |
|
CB1 | Patch clamp | Inhibition | 23142606 | |||||||
| TRPV1 |
|
CDK5 | In vitro PTM assay | Phosphorylation | 22451679 | |||||||
| TRPV1 |
|
CDK5 | In vitro PTM assay | Phosphorylation (Thr-407) | 17194758 | |||||||
| TRPV1 |
|
CDK5 | In vivo PTM assay | Phosphorylation (Thr-407) | 17194758 | |||||||
| TRPV1 |
|
FAF1 | Calcium measurement | Inhibition | 16510717 | |||||||
| TRPV1 |
|
FAF1 | Patch clamp | Inhibition | 16510717 | |||||||
| TRPV1 |
|
GABARAP | Calcium measurement | Inhibition | 20179142 | |||||||
| TRPV1 |
|
GABARAP | Cell surface biotinylation | Increase in plasma membrane level | 20179142 | |||||||
| TRPV1 |
|
GABARAP | Patch clamp | Inhibition | 20179142 | |||||||
| TRPV1 |
|
PIRT | Patch clamp | Activation | 18455988 | |||||||
| TRPV1 |
|
PKA | In vitro PTM assay | Phosphorylation (Ser-116) | 12194871 | |||||||
| TRPV1 |
|
PKA | Patch clamp | Activation | 12194871 | |||||||
| TRPV1 |
|
PKC | In vitro PTM assay | Phosphorylation | 14523239 | |||||||
| TRPV1 |
|
PKCε | In vitro PTM assay | Phosphorylation (Ser-800) | 11884385 | |||||||
| TRPV1 |
|
PKCε | In vivo PTM assay | Phosphorylation (Ser-502) | 11884385 | |||||||
| TRPV1 |
|
PKCε | Patch clamp | Activation | 11884385 | |||||||
| TRPV1 |
|
PRKD1/PKCμ | Calcium measurement | Activation | 15471852 | |||||||
| TRPV1 |
|
PRKD1/PKCμ | In vitro PTM assay | Phosphorylation (Ser-116) | 15471852 | |||||||
| TRPV1 |
|
SRC | Calcium measurement | Activation | 16319926 | |||||||
| TRPV1 |
|
SRC | In vitro PTM assay | Phosphorylayion (Tyr-200) | 16319926 | |||||||
| TRPV1 |
|
SRC | In vivo PTM assay | Phosphorylation | 15084474 | |||||||
| TRPV1 |
|
SRC | In vivo PTM assay | Phosphorylayion (Tyr-200) | 16319926 | |||||||
| TRPV1 |
|
SRC | Patch clamp | Activation | 15084474 | |||||||
| TRPV1 |
|
SRC | Patch clamp | Activation | 16319926 | |||||||
| TRPV1 |
|
TrkA | In vivo PTM assay | Phosphorylayion (Tyr-200) | 16319926 | |||||||
| TRPV1 |
|
TrkA | Patch clamp | Activation | 16319926 | |||||||
| TRPV1 |
|
β-arrestin 2 | Calcium measurement | Desensitization | 22952227 | |||||||
| TRPV1 |
|
β-arrestin 2 | In vivo PTM assay | Dephosphorylation | 22952227 | |||||||
(:
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:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)