TRPV5 / interactors
- At a glance
- In detail
Name: Transient receptor potential cation channel subfamily V member 5
(TRPV5)
Official Symbol: TRPV5
provided by HGNC
Function:
Constitutively active calcium selective cation channel thought to be involved in Ca(2+) reabsorption in kidney and intestine. The channel is activated by low internal calcium level and the current exhibits an inward rectification. A Ca(2+)-dependent feedback regulation includes fast channel inactivation and slow current decay. Heteromeric assembly with TRPV6 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating (By similarity).
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt)
Nucleic Acids Res. 40: D71-D75 (2012).
| Species |
External DB
|
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|---|---|---|---|---|---|---|---|---|---|---|
| Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM | |
Human
 |
56302 |
Q9NQA5 |
DIP-43887N |
Q9NQA5 |
MINT-1470011 |
121137 |
Q9NQA5 |
511 |
hsa:56302 |
606679 |
|
Mouse
|
194352 |
P69744 |
DIP-34874N |
P69744 |
228781 |
P69744 |
mmu:194352 |
|||
|
Rat
|
116469 |
Q9JIP0 |
MINT-5159302 |
250447 |
Q9JIP0 |
rno:116469 |
||||
PPI pairs:
Biological Process:
- GO:0051262
: protein tetramerization
Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
World Wide Web URL: http://www.geneontology.org/
Disease:
No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine,
Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/
World Wide Web URL: http://omim.org/
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
Screening
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|---|---|---|---|---|---|---|---|---|---|---|
| Experimental screening | Non-experimental screening | Reference | ||||||||
| TRP channel construct | Interactor source | |||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | |||
| TRPV5 |
|
80K-H | Inference | Prediction | 15100231 | |||||
| TRPV5 |
|
BSPRY | Inference | Prediction | 16380433 | |||||
| TRPV5 |
|
Calbindin-D28K | Inference | Prediction | 16763551 | |||||
| TRPV5 |
|
Calmodulin | Inference | Prediction | 15123711 | |||||
| TRPV5 |
|
Cyclophilin B | Inference | Prediction | 18445599 | |||||
| TRPV5 |
|
EWSR1 | Yeast two-hybrid | Human | Not specified | Human | Not specified | Not specified | 16189514 | |
| TRPV5 |
|
FKBP52/FKBP4 | Inference | Prediction | 16352746 | |||||
| TRPV5 |
|
Klotho | Inference | Prediction | 16239475 | |||||
| TRPV5 |
|
NHERF2 | Inference | Prediction | 15665527 | |||||
| TRPV5 |
|
NHERF4 | Yeast two-hybrid | Mouse | C-terminus | Mouse | Kidney | cDNA library | 16565876 | |
| TRPV5 |
|
Nipsnap1 | Inference | Prediction | 18392847 | |||||
| TRPV5 |
|
Rab11a | Inference | Prediction | 16354700 | |||||
| TRPV5 |
|
S100A10 | Yeast two-hybrid | Mouse | C-terminus | Mouse | Kidney | cDNA library | 12660155 | |
| TRPV5 |
|
TGM2 | Inference | Prediction | 21952826 | |||||
| TRPV5 |
|
TRPV6 | Inference | Prediction | 12574114 | |||||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Validation: In vitro validation
|
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|---|---|---|---|---|---|---|---|---|---|---|
| Assay with recombinant proteins | Reference | |||||||||
| TRP channel construct | Interactor construct | |||||||||
| TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | ||
| TRPV5 |
|
80K-H | Fusion protein-pull down assay | Mouse | C-terminus | E. coli | Mouse | Full-length | In vitro translation | 15100231 |
| TRPV5 |
|
BSPRY | Fusion protein-pull down assay | Mouse | 591-595 | E. coli | Mouse | Full-length | Xenopus oocyte lysates | 16380433 |
| TRPV5 |
|
BSPRY | Fusion protein-pull down assay | Mouse | Full-length | In vitro translation | Mouse | Full-length | E. coli | 16380433 |
| TRPV5 |
|
Calbindin-D28K | Fusion protein-pull down assay | Mouse | N-terminus | E. coli | Not specified | Full-length | In vitro translation | 16763551 |
| TRPV5 |
|
Calbindin-D28K | Fusion protein-pull down assay | Mouse | C-terminus | E. coli | Not specified | Full-length | In vitro translation | 16763551 |
| TRPV5 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | Full-length | In vitro translation | Not specified | Full-length | Not specified | 15123711 |
| TRPV5 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | 1-327 | In vitro translation | Not specified | Full-length | Not specified | 15123711 |
| TRPV5 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | 578-730 | In vitro translation | Not specified | Full-length | Not specified | 15123711 |
| TRPV5 |
|
FKBP52/FKBP4 | Fusion protein-pull down assay | Mouse | Full-length | In vitro translation | Mouse | Full-length | E. coli | 16352746 |
| TRPV5 |
|
NHERF2 | Fusion protein-pull down assay | Mouse | Full-length | In vitro translation | Mouse | Full-length | E. coli | 15665527 |
| TRPV5 |
|
NHERF2 | Fusion protein-pull down assay | Mouse | Full-length | E. coli | Not specified | Full-length | In vitro translation | 16565876 |
| TRPV5 |
|
NHERF4 | Fusion protein-pull down assay | Mouse | C-terminus | E. coli | Mouse | Full-length | In vitro translation | 16565876 |
| TRPV5 |
|
Rab11a | Fusion protein-pull down assay | Mouse | Full-length | E. coli | Mouse | Full-length | In vitro translation | 16354700 |
| TRPV5 |
|
S100A10 | Fusion protein-pull down assay | Mouse | Full-length | E. coli | Mouse | Full-length | Xenopus oocyte lysates | 12660155 |
| TRPV5 |
|
TGM2 | Fusion protein-pull down assay | Mouse | Full-length | HEK293 lysates | Guinea pig | Not specified | Guinea pig liver | 21952826 |
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Validation: In vivo validation
|
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|---|---|---|---|---|---|---|---|---|---|---|
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference | ||||||||
| Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | |||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
| TRPV5 |
|
80K-H | Co-immunofluorescence staining | Mouse kidney section | 15100231 | |||||
| TRPV5 |
|
80K-H | Co-immunoprecipitation | Xenopus oocyte | Mouse | Full-length | Mouse | Full-length | 15100231 | |
| TRPV5 |
|
BSPRY | Co-immunofluorescence staining | Mouse kidney section | 16380433 | |||||
| TRPV5 |
|
BSPRY | Co-immunoprecipitation | MDCK | Mouse | Full-length | Mouse | Full-length | 16380433 | |
| TRPV5 |
|
Calbindin-D28K | Co-immunofluorescence staining | Mouse kidney section | 16763551 | |||||
| TRPV5 |
|
Calbindin-D28K | Co-immunoprecipitation | HEK293 | Mouse | Full-length | Not specified | Full-length | 16763551 | |
| TRPV5 |
|
FKBP52/FKBP4 | Co-immunofluorescence staining | Mouse kidney section | 16352746 | |||||
| TRPV5 |
|
FKBP52/FKBP4 | Co-immunoprecipitation | HEK293 | Mouse | Full-length | Not used | 16352746 | ||
| TRPV5 |
|
Klotho | Co-immunofluorescence staining | Mouse kidney section | 16239475 | |||||
| TRPV5 |
|
NHERF4 | Co-immunofluorescence staining | Xenopus oocyte | Mouse | Full-length | Mouse | Full-length | 16565876 | |
| TRPV5 |
|
NHERF4 | Co-immunofluorescence staining | Mouse kidney section | 16565876 | |||||
| TRPV5 |
|
NHERF4 | Co-immunoprecipitation | HEK293 | Mouse | Full-length | Mouse | Full-length | 16565876 | |
| TRPV5 |
|
Nipsnap1 | Co-immunoprecipitation | HEK293 | Rabbit | Full-length | Not specified | Full-length | 18392847 | |
| TRPV5 |
|
Rab11a | Co-immunofluorescence staining | Xenopus oocyte | Mouse | Full-length | Mouse | Full-length | 16354700 | |
| TRPV5 |
|
Rab11a | Co-immunofluorescence staining | Mouse kidney section | 16354700 | |||||
| TRPV5 |
|
Rab11a | Co-immunoprecipitation | Xenopus oocyte | Mouse | Full-length | Mouse | Full-length | 16354700 | |
| TRPV5 |
|
S100A10 | Co-immunofluorescence staining | Rat duodenum tissue | 12660155 | |||||
| TRPV5 |
|
TRPV6 | Co-immunofluorescence staining | Mouse kidney section | 12574114 | |||||
| TRPV5 |
|
TRPV6 | Co-immunoprecipitation | HEK293 | Mouse | Full-length | Mouse | Full-length | 15713749 | |
| TRPV5 |
|
TRPV6 | Co-immunoprecipitation | Human myeloid leukemia K562 cell | 19295174 | |||||
| TRPV5 |
|
TRPV6 | Co-immunoprecipitation | Xenopus oocyte | Rat | Full-length | Mouse | Full-length | 12574114 | |
| TRPV5 |
|
TRPV6 | Fluorescence resonance energy transfer | HEK293 | Mouse | Full-length | Mouse | Full-length | 15713749 | |
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Characterization
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | |||||||
| TRP channel | Interactor | |||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
| TRPV5 |
|
80K-H | Fusion protein-pull down assay | Mouse | 598-608 | Mouse | Not determined | 15100231 | ||
| TRPV5 |
|
Calmodulin | Fluorescence probe labeling | Human | 587-616 | Not specified | Not determined | 306 nM | 20686800 | |
| TRPV5 |
|
Calmodulin | Fusion protein-pull down assay | Human | ARD domain | Not specified | Not determined | 20926660 | ||
| TRPV5 |
|
Calmodulin | Fusion protein-pull down assay | Human | 696-729 | Not specified | Not determined | 21576356 | ||
| TRPV5 |
|
Calmodulin | Native-gel electrophoresis | Human | 696-729 | Not specified | Not determined | 21576356 | ||
| TRPV5 |
|
NHERF2 | Fusion protein-pull down assay | Mouse | C-terminus | Mouse | Not determined | 15665527 | ||
| TRPV5 |
|
NHERF4 | Fusion protein-pull down assay | Mouse | 579-617 | Mouse | Not determined | 16565876 | ||
| TRPV5 |
|
Rab11a | Fusion protein-pull down assay | Mouse | 596-600 | Mouse | Not determined | 16354700 | ||
| TRPV5 |
|
S100A10 | Fusion protein-pull down assay | Mouse | 591-595 | Mouse | Not determined | 12660155 | ||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Functional consequence
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | ||||||
| TRPV5 |
|
BSPRY | Calcium measurement | Inhibition | 16380433 | |||||||
| TRPV5 |
|
Calbindin-D28K | Patch clamp | Activation | 16763551 | |||||||
| TRPV5 |
|
Calmodulin | Calcium measurement | Inhibition | 21576356 | |||||||
| TRPV5 |
|
Calmodulin | Patch clamp | Inhibition | 21576356 | |||||||
| TRPV5 |
|
Cyclophilin B | Calcium measurement | Activation | 18445599 | |||||||
| TRPV5 |
|
FKBP52/FKBP4 | Calcium measurement | Inhibition | 16352746 | |||||||
| TRPV5 |
|
FKBP52/FKBP4 | Patch clamp | Inhibition | 16352746 | |||||||
| TRPV5 |
|
Klotho | Calcium measurement | Activation | 16239475 | |||||||
| TRPV5 |
|
Klotho | Calcium measurement | Activation | 18495742 | |||||||
| TRPV5 |
|
Klotho | Cell surface biotinylation | Increase in plasma membrane level | 16239475 | |||||||
| TRPV5 |
|
Klotho | In vitro PTM assay | Glycosylation (Asn-358) | 16239475 | |||||||
| TRPV5 |
|
Klotho | Patch clamp | Activation | 16239475 | |||||||
| TRPV5 |
|
Rab11a | Calcium measurement | Activation | 16354700 | |||||||
| TRPV5 |
|
Rab11a | Co-immunofluorescence staining | Increase in plasma membrane level | 16354700 | |||||||
| TRPV5 |
|
S100A10 | Co-immunofluorescence staining | Increase in plasma membrane level | 12660155 | |||||||
| TRPV5 |
|
S100A10 | Patch clamp | Activation | 12660155 | |||||||
| TRPV5 |
|
TGM2 | Calcium measurement | Inhibition | 21952826 | |||||||
| TRPV5 |
|
TGM2 | Cell surface biotinylation | Decrease in plasma membrane level | 21952826 | |||||||
| TRPV5 |
|
TGM2 | Patch clamp | Inhibition | 21952826 | |||||||
| TRPV5 |
|
TRPV6 | Patch clamp | New channel creation | 12574114 | |||||||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)