Neuron. 2001 Mar;29(3):645-55.

TRPC1 and TRPC5 form a novel cation channel in mammalian brain.External

Strubing, C., Krapivinsky, G., Krapivinsky, L., Clapham, D. E.,
["Howard Hughes Medical Institute, Cardiovascular Research, Children's Hospital, 320 Longwood Avenue, Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA."]
TRP proteins are cation channels responding to receptor-dependent activation of phospholipase C. Mammalian (TRPC) channels can form hetero-oligomeric channels in vitro, but native TRPC channel complexes have not been identified to date. We demonstrate here that TRPC1 and TRPC5 are subunits of a heteromeric neuronal channel. Both TRPC proteins have overlapping distributions in the hippocampus. Coexpression of TRPC1 and TRPC5 in HEK293 cells resulted in a novel nonselective cation channel with a voltage dependence similar to NMDA receptor channels, but unlike that of any reported TRPC channel. TRPC1/TRPC5 heteromers were activated by G(q)-coupled receptors but not by depletion of intracellular Ca(2+) stores. In contrast to the more common view of the TRP family as comprising store-operated channels, we propose that many TRPC heteromers form diverse receptor-regulated nonselective cation channels in the mammalian brain.
PMID: 11301024External
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Screening Toggle
  Experimental screening Non-experimental screening Reference
TRP channel construct Interactor source
TRP channel Interactor Method Species Region Species Organ/tissue Sample type
TRPC1 Link TRPC4 Inference Prediction 11301024
TRPC1 Link TRPC5 Inference Prediction 11301024
TRPC4 Link TRPC1 Inference Prediction 11301024
TRPC5 Link TRPC1 Inference Prediction 11301024
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Validation: In vivo validation Toggle
  Assay with endogenous proteins Assay with overexpressed proteins Reference
Cell or tissue Cell or tissue TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Species Region
TRPC1 Link TRPC4 Co-immunoprecipitation Rat brain microsome 11301024
TRPC1 Link TRPC5 Co-immunoprecipitation Rat brain microsome 11301024
TRPC1 Link TRPC5 Co-immunoprecipitation HEK293 Human Full-length Mouse Full-length 11301024
TRPC4 Link TRPC1 Co-immunoprecipitation Rat brain microsome 11301024
TRPC5 Link TRPC1 Co-immunoprecipitation Rat brain microsome 11301024
TRPC5 Link TRPC1 Co-immunoprecipitation HEK293 Mouse Full-length Human Full-length 11301024
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Functional consequence Toggle
TRP channel Interactor Method Post-translational modification Subcellular trafficking Activity Reference
TRPC1 Link TRPC4 Patch clamp New channel creation 11301024
TRPC1 Link TRPC5 Patch clamp New channel creation 11301024
TRPC4 Link TRPC1 Patch clamp New channel creation 11301024
TRPC5 Link TRPC1 Patch clamp New channel creation 11301024
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
TRP / Interactor

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