Nat Cell Biol. 2002 May;4(5):329-36.

The TRPM7 channel is inactivated by PIP(2) hydrolysis.External

Runnels, L. W., Yue, L., Clapham, D. E.,
["Howard Hughes Medical Institute, Children's Hospital, Harvard Medical School, Enders 1309, 320 Longwood Avenue, Boston, MA 02115, USA."]
TRPM7 (ChaK1, TRP-PLIK, LTRPC7) is a ubiquitous, calcium-permeant ion channel that is unique in being both an ion channel and a serine/threonine kinase. The kinase domain of TRPM7 directly associates with the C2 domain of phospholipase C (PLC). Here, we show that in native cardiac cells and heterologous expression systems, G alpha q-linked receptors or tyrosine kinase receptors that activate PLC potently inhibit channel activity. Numerous experimental approaches demonstrated that phosphatidylinositol 4,5-bisphosphate (PIP(2)), the substrate of PLC, is a key regulator of TRPM7. We conclude that receptor-mediated activation of PLC results in the hydrolysis of localized PIP(2), leading to inactivation of the TRPM7 channel.
PMID: 11941371External
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Screening Toggle
  Experimental screening Non-experimental screening Reference
TRP channel construct Interactor source
TRP channel Interactor Method Species Region Species Organ/tissue Sample type
TRPM7 Link PLCβ2 Inference Prediction 11941371
TRPM7 Link PLCβ3 Inference Prediction 11941371
TRPM7 Link PLCγ1 Inference Prediction 11941371
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Validation: In vitro validation Toggle
  Assay with recombinant proteins Reference
TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Expression system Species Region Expression system
TRPM7 Link PLCβ1 Fusion protein-pull down assay Rat 1352-1862 E. coli Rat Full-length HEK293T lysates 11941371
TRPM7 Link PLCβ1 Yeast two-hybrid Rat 1532-1862 Yeast Rat 643-1216 Yeast 11941371
TRPM7 Link PLCβ2 Fusion protein-pull down assay Rat 1352-1862 E. coli Rat Full-length HEK293T lysates 11941371
TRPM7 Link PLCβ3 Fusion protein-pull down assay Rat 1352-1862 E. coli Rat Full-length HEK293T lysates 11941371
TRPM7 Link PLCγ1 Fusion protein-pull down assay Rat 1352-1862 E. coli Rat Full-length HEK293T lysates 11941371
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Validation: In vivo validation Toggle
  Assay with endogenous proteins Assay with overexpressed proteins Reference
Cell or tissue Cell or tissue TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Species Region
TRPM7 Link PLCβ2 Co-immunoprecipitation HEK293T Rat Full-length Rat Full-length 11941371
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Characterization Toggle
  Binding region mapping Stoichiometry Affinity (Kd) Reference
TRP channel Interactor
TRP channel Interactor Method Species Region Species Region
TRPM7 Link PLCβ1 Yeast two-hybrid Rat 1532-1862 Rat 643-797 11941371
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Functional consequence Toggle
TRP channel Interactor Method Post-translational modification Subcellular trafficking Activity Reference
TRPM7 Link PLCβ1 Patch clamp Inhibition 11941371
TRPM7 Link PLCβ2 Patch clamp Inhibition 11941371
TRPM7 Link PLCβ3 Patch clamp Inhibition 11941371
TRPM7 Link PLCγ1 Patch clamp Inhibition 11941371
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
TRP / Interactor

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