EMBO Rep. 2008 May;9(5):472-9. Epub 2008 Mar 7.

Formation of a new receptor-operated channel by heteromeric assembly of TRPP2 and TRPC1 subunits.External

Bai, C. X., Giamarchi, A., Rodat-Despoix, L., Padilla, F., Downs, T., Tsiokas, L., Delmas, P.,
["University of Oklahoma Health Sciences Center, 975 NE 10th Street, Oklahoma City, Oklahoma 73104, USA."]
Although several protein-protein interactions have been reported between transient receptor potential (TRP) channels, they are all known to occur exclusively between members of the same group. The only intergroup interaction described so far is that of TRPP2 and TRPC1; however, the significance of this interaction is unknown. Here, we show that TRPP2 and TRPC1 assemble to form a channel with a unique constellation of new and TRPP2/TRPC1-specific properties. TRPP2/TRPC1 is activated in response to G-protein-coupled receptor activation and shows a pattern of single-channel conductance, amiloride sensitivity and ion permeability distinct from that of TRPP2 or TRPC1 alone. Native TRPP2/TRPC1 activity is shown in kidney cells by complementary gain-of-function and loss-of-function experiments, and its existence under physiological conditions is supported by colocalization at the primary cilium and by co-immunoprecipitation from kidney membranes. Identification of the heteromultimeric TRPP2/TRPC1 channel has implications in mechanosensation and cilium-based Ca(2+) signalling.
PMID: 18323855External
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Validation: In vivo validation Toggle
  Assay with endogenous proteins Assay with overexpressed proteins Reference
Cell or tissue Cell or tissue TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Species Region
TRPC1 Link TRPP1 Co-immunoprecipitation Rat kidney tissue 18323855
TRPC1 Link TRPP1 Co-immunofluorescence staining mIMCD-3 18323855
TRPP1 Link TRPC1 Co-immunoprecipitation Rat kidney tissue 18323855
TRPP1 Link TRPC1 Co-immunofluorescence staining mIMCD-3 18323855
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Functional consequence Toggle
TRP channel Interactor Method Post-translational modification Subcellular trafficking Activity Reference
TRPC1 Link TRPP1 Patch clamp New channel creation 18323855
TRPP1 Link TRPC1 Patch clamp New channel creation 18323855
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
TRP / Interactor

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