EMBO Rep. 2008 Jul;9(7):690-7. Epub 2008 Jun 6.

Off-response property of an acid-activated cation channel complex PKD1L3-PKD2L1.External

Inada, H., Kawabata, F., Ishimaru, Y., Fushiki, T., Matsunami, H., Tominaga, M.,
["Section of Cell Signaling, Okazaki Institute for Integrative Bioscience, Higashiyama 5-1, Myodaiji, Okazaki, Aichi 444-8787, Japan. hinada@nips.ac.jp"]
Ligand-gated ion channels are important in sensory and synaptic transduction. The PKD1L3-PKD2L1 channel complex is a sour taste receptor candidate that is activated by acids. Here, we report that the proton-activated PKD1L3-PKD2L1 ion channels have the unique ability to be activated after the removal of an acid stimulus. We refer to this property as the off-response (previously described as a delayed response). Electrophysiological analyses show that acid-induced responses are observed only after the removal of an acid solution at less than pH 3.0. A small increase in pH is sufficient for PKD1L3-PKD2L1 channel activation, after exposure to an acid at pH 2.5. These results indicate that this channel is a new type of ion channel-designated as an 'off-channel'-which is activated during stimulus application but not gated open until the removal of the stimulus. The off-response property of PKD1L3-PKD2L1 channels might explain the physiological phenomena occurring during sour taste sensation.
PMID: 18535624External
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Validation: In vivo validation Toggle
  Assay with endogenous proteins Assay with overexpressed proteins Reference
Cell or tissue Cell or tissue TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Species Region
TRPP2 Link PKD1L3/Polycystin-1L3 Co-immunoprecipitation HEK293T Mouse Full-length Mouse Full-length 18535624
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Functional consequence Toggle
TRP channel Interactor Method Post-translational modification Subcellular trafficking Activity Reference
TRPP2 Link PKD1L3/Polycystin-1L3 Patch clamp New channel creation 18535624
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TRP / Interactor

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