Cell Signal. 2012 Mar;24(3):641-51. Epub 2011 Nov 10.

Phosphorylation on the Ser 824 residue of TRPV4 prefers to bind with F-actin than with microtubules to expand the cell surface area.External

Shin, S. H., Lee, E. J., Hyun, S., Chun, J., Kim, Y., Kang, S. S.,
["Department of Biology Education, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea."]
Previously, we demonstrated that the transient receptor potential vanilloid 4 (TRPV4) cation channel, a member of the TRP vanilloid subfamily, is one of the serum glucocorticoid-induced protein kinase1 (SGK1) authentic substrate proteins, and that the Ser 824 residue of TRPV4 is phosphorylated by SGK1. In this study, we demonstrated that phosphorylation on the Ser 824 residue of TRPV4 is required for its interaction with F-actin, using TRPV4 mutants (S824D; a phospho-mimicking TRPV4 mutant and S824A; a non-phosphorylatable TRPV4 mutant) and its proper subcellular localization. Additionally, we noted that the phosphorylation of the Ser824 residue promotes its single channel activity, Ca(2+) influx, protein stability, and cell surface area (expansion of plasma membrane).
PMID: 22101010External
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Screening Toggle
  Experimental screening Non-experimental screening Reference
TRP channel construct Interactor source
TRP channel Interactor Method Species Region Species Organ/tissue Sample type
TRPV4 Link γ-actin Inference Prediction 22101010
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
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Validation: In vitro validation Toggle
  Assay with recombinant proteins Reference
TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Expression system Species Region Expression system
TRPV4 Link Tubulin Fusion protein-pull down assay Mouse Full-length E. coli Not used HEK293 lysates 22101010
TRPV4 Link γ-actin Fusion protein-pull down assay Mouse Full-length E. coli Not used HEK293 lysates 22101010
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
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Validation: In vivo validation Toggle
  Assay with endogenous proteins Assay with overexpressed proteins Reference
Cell or tissue Cell or tissue TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Species Region
TRPV4 Link Tubulin Co-immunoprecipitation HEK293 Mouse Full-length Not used 22101010
TRPV4 Link γ-actin Co-immunoprecipitation HEK293 Mouse Full-length Not used 22101010
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 Screening Arrow2 Validation: In vitro validation Arrow2 Validation: In vivo validation Arrow2 Characterization Arrow2 Functional consequence Arrow2 top
Characterization Toggle
  Binding region mapping Stoichiometry Affinity (Kd) Reference
TRP channel Interactor
TRP channel Interactor Method Species Region Species Region
TRPV4 Link Tubulin Fusion protein-pull down assay Mouse 718-871 Human Not determined 22101010
TRPV4 Link γ-actin Fusion protein-pull down assay Mouse 718-871 Human Not determined 22101010
(Link: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
TRP / Interactor

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